. Author manuscript; available in PMC: 2012 Nov 15.

Published in final edited form as: Biochemistry. 2011 Oct 14;50(45):9694–9707. doi: 10.1021/bi201276r

Table 2.

Kinetic parameters determined from measuring the initial rates of pyruvate carboxylation with varying concentrations of pyruvate at fixed concentrations of free Mg²⁺^a.

	V_max (μmol mg⁻¹ min⁻¹)	K_{m pyruvate} (mM)	V/K_{m pyruvate}
0.7 mM Mg²⁺	0.69 ± 0.02	3.1 ± 0.2	0.22 ± 0.03
1.5 mM Mg²⁺	1.41 ± 0.01	3.0 ± 0.4	0.47 ± 0.08
3.0 mM Mg²⁺	3.18 ± 0.08	2.65 ± 0.04	1.2 ± 0.7
5.0 mM Mg²⁺	3.98 ± 0.04	0.15 ± 0.01	27.3 ± 0.5

Kinetic parameters were determined from data fits to eqn (1) or eqn (3).