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. 1982 Aug 11;10(15):4595–4604. doi: 10.1093/nar/10.15.4595

Adaptive increase of O6-methylguanine-acceptor protein in HeLa cells following N-methyl-N'-nitro-N-nitrosoguanidine treatment.

E A Waldstein, E H Cao, R B Setlow
PMCID: PMC321114  PMID: 7133992

Abstract

We have assayed in extracts of HeLa cells the amount of acceptor protein that removes O6-methylguanine adducts from alkylated DNA. Cells were treated with single or multiple nontoxic doses of N-methyl-N'-nitrosoguanidine (MNNG) and the extracts were analyzed up to 32 h after the last exposure. The acceptor activity assayed immediately (1 h) after single exposures decreases linearly with dose indicating that the acceptor protein is used up by endogenous O6-methylguanine adducts in a stoichiometric reaction. Multiple exposures, assayed 8-24 h after the last exposure, increase the amount of acceptor protein in a dose dependent fashion followed by a decrease above a cumulative dose of 100 ng/ml. Under conditions of maximum induction, there are about 300,000 acceptor protein sites per cell, approximately 3 fold above the constitutive level. Both in adapted and unadapted cells the methyl group from O6-methylguanine adducts in the alkylated DNA is transferred to cysteine residues of the acceptor protein(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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