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Developmental expression of matA at the resident locus. Expression of matA was analyzed in undifferentiated hyphae, H, and in reproductive tissue 6 days postinduction of sexual development, D. Expression of the matA transgene at the matA(0) locus is suppressed in the reproductive tissue (before 5′-FOA). Wild-type expression of matA is restored at the resident locus (after 5′-FOA) upon removal of matA(0) and plasmid sequences and the recovery of wild-type genomic structure.
