Realistic 3D Computer Model of the Gerbil Middle Ear, Featuring Accurate Morphology of Bone and Soft Tissue Structures

Abstract

In order to improve realism in middle ear (ME) finite-element modeling (FEM), comprehensive and precise morphological data are needed. To date, micro-scale X-ray computed tomography (μCT) recordings have been used as geometric input data for FEM models of the ME ossicles. Previously, attempts were made to obtain these data on ME soft tissue structures as well. However, due to low X-ray absorption of soft tissue, quality of these images is limited. Another popular approach is using histological sections as data for 3D models, delivering high in-plane resolution for the sections, but the technique is destructive in nature and registration of the sections is difficult. We combine data from high-resolution μCT recordings with data from high-resolution orthogonal-plane fluorescence optical-sectioning microscopy (OPFOS), both obtained on the same gerbil specimen. State-of-the-art μCT delivers high-resolution data on the 3D shape of ossicles and other ME bony structures, while the OPFOS setup generates data of unprecedented quality both on bone and soft tissue ME structures. Each of these techniques is tomographic and non-destructive and delivers sets of automatically aligned virtual sections. The datasets coming from different techniques need to be registered with respect to each other. By combining both datasets, we obtain a complete high-resolution morphological model of all functional components in the gerbil ME. The resulting 3D model can be readily imported in FEM software and is made freely available to the research community. In this paper, we discuss the methods used, present the resulting merged model, and discuss the morphological properties of the soft tissue structures, such as muscles and ligaments.

Introduction

The middle ear (ME) forms a small 3D biomechanical system. It mainly consists of the tympanic membrane (TM), three ossicles—malleus, incus, and stapes—and their supporting ligaments and muscles. The remarkable performance of ME mechanics is too complex to be understood intuitively. For better understanding, ME modeling was introduced. Finite-element (computer) modeling (FEM) has become an established numerical technique to simulate ME mechanics. In ME research, the technique was first introduced by Funnell and Laszlo (1978). As one of its inputs, FEM requires 3D morphological computer models of the ME components. These mesh models consist of a finite number of elements, e.g., tetrahedra or hexahedra.

Current morphological models are either incomplete, low resolution, and/or contain rudimentary shapes to represent (some) ME components. Pioneering work in this field used manually drawn geometrical shapes in the computer to represent the ME malleus, incus, and stapes (Wada et al. 1992; Ladak and Funnell 1996; Blayney et al. 1997; Eiber et al. 2000; Prendergast et al. 2000; Koike et al. 2002). Some authors used low- or modest-resolution shapes measured with medical X-ray computed tomography (CT) (Rodt et al. 2002; Lee et al. 2006) or with tabletop micro-CT (μCT) devices (Decraemer et al. 2002, 2003; Elkhouri et al. 2006; Puria and Steele 2010; Lee et al. 2010). Other authors used histological sectioning (Funnell et al. 1992; Sun et al. 2002) or magnetic resonance microscopy (MRM, NMR, MRI) (Funnell et al. 2005; Elkhouri et al. 2006), but again with modest resolutions. In many models, the suspensory ligaments and muscle tendons are either omitted (Wada et al. 1992; Ladak and Funnell 1996; Blayney et al. 1997; Lord et al. 1999; Rodt et al. 2002) or manually incorporated as simple geometrical objects such as blocks, cylinders, or cones (Prendergast et al. 2000; Beer et al. 2000; Koike et al. 2002; Sun et al. 2002; Lee et al. 2006). To the authors’ knowledge, only models by Wang et al. (2006), Gan et al. (2007), and Cheng and Gan (2008) (using histological sectioning) and by Mikhael et al. (2004), Sim and Puria (2008), and Ruf et al. 2009 (using X-ray techniques) contain actually measured shapes of soft tissue structures, but in low resolution.

To improve realism in FEM calculations, ME geometry models need to incorporate all and accurate shapes of the ossicles and suspensory soft tissue structures (Decraemer et al. 2003). As the computer calculating capacity has grown to a point where it can manage large amounts of data and as the scientific measurement apparatus is now capable of high-resolution imaging on all kinds of tissue types, the time has come to incorporate realistic and complete morphological 3D ME models in FEM. We point out that it might not be necessary, even not numerically feasible, to perform FEM with all structures described in the highest detail. On the other hand, it is difficult to decide beforehand how precise the morphologic model needs to be. Therefore, we think it is important to first have a high-resolution morphologic model available, which can then be simplified to the modeler’s judgment.

In the current paper, we provide these high-quality models by combining data originating from two different tomographic techniques: State-of-the-art μCT tomography allows to obtain precise data on bony structures, but due to the low X-ray absorption of soft tissue, CT generates poor quality images of soft tissue (Lemmerling et al. 1997). Therefore, we combine these data with measurements from another and relatively new technique: orthogonal-plane fluorescence optical-sectioning (OPFOS) microscopy or tomography. This method images both bone and soft tissue at the same time and in high resolution. As gerbil is one of the standard laboratory animal models in fundamental hearing research, we chose this species for our first model.

Materials and methods

Dissection

All animal manipulations in this work were performed in accordance with Belgian legislation and the directives set by the Ethical committee on Animal Experimentation of our institution (University of Antwerp, Belgium). Three adult Mongolian gerbils (Meriones unguiculatus), aged between 3 and 6 months, were used. They were housed in cages with food and water ad libitum in our animal facility.

The animals were euthanized using carbon dioxide, followed by a cardiac perfusion with physiological fluid to rinse out all the blood from the gerbil head blood vessels. This step is necessary to allow for OPFOS tomography (as we will explain below). The gerbils are then decapitated and the right temporal bones were isolated. The specimens were reduced in size until only the bulla was left containing the middle and inner ear, cf. Figure 1. During the harvesting of these bullas, continuous moistening with mist from an ultrasonic humidifier (Bionaire BT-204) was applied to avoid dehydration.

FIG. 1.

3D model of separate surface meshes of bony middle and inner ear components of gerbil 2, obtained from μCT. The bulla is rendered transparent. Voxel size is 8.5 × 8.5 × 8.5 μm.

Cross-sectional imaging of bone with X-ray tomography

The first stage of 3D tomographic recording of the ME was achieved using micro-scale X-ray computed tomography. The dissected bullae were enclosed in separate Eppendorf vials, together with a calibration object and a few droplets of physiological fluid at the bottom. In this way, a 100% saturated humid environment was created to avoid dehydration artifacts. Another droplet of fluid was placed in the ear canal—which could help—to distinguish the outline border of the TM shape with the air-filled ME cavity. Water and air have a slightly different X-ray absorption coefficient, so a layer of water on the extremely thin TM can help to reveal its medial shape outline. In previous work, we measured the shape of the eardrum before and after putting fluid on the membrane: Even with a 10 mm water column in the ear canal, no measurable deformation was found with moiré profilometry of 15 μm resolution (Buytaert et al. 2009). As the droplet of water is less than 3 mm high (inducing a pressure load of 30 Pa), the TM deformation is well below the μCT measurement resolution. The Eppendorf vials (made from polypropylene) are almost X-ray transparent. Especially bone absorbs X-rays well, thus creating a high contrast in transmission recordings. The small calibration objects were custom-made from polyvinyl chloride in our mechanical workshop and possess about the same X-ray absorption properties as thin bone (Gea et al. 2005). They served as an independent calibration to verify the μCT device specifications.

The vials containing gerbil specimens were scanned at the UGCT scanning facility at Ghent University (www.ugct.ugent.be) using a custom-built μCT scanner of medium energy (up to 160 keV). The scanner has a directional X-ray tube with a feature recognition capability up to 2 μm (Masschaele et al. 2007). The scans were performed at a tube voltage of 120 kV (photon energy levels ranging from 0 to 120 keV) and a current of 58 μA. A custom-made vial holder was mounted on a computer-controlled rotation table (MICOS, UPR160F-AIR). For each specimen, a series of 1,000 shadow projections of 1,496 × 1,880 pixels was recorded covering an object rotation of 360° (or one recording every 0.36°). Reconstruction of the tomographic data volume to serial sections was achieved using the back-projection algorithms of the Octopus software package (Dierick et al. 2004), resulting in 1,780 reconstructed cross sections of 1,496 × 1,496 pixels. From these calculated cross sections with an isometric pixel size of 8.5 μm, accurate 3D models of the three ossicles and other bony structures were generated. All three datasets cover a volume of 15.1 × 12.7 × 12.7 mm (1,780 × 1,496 × 1,496 × 8.5 μm).

Cross-sectional imaging of soft tissue with optical tomography

Due to the low X-ray absorption of soft tissue, another tomographic technique was needed: OPFOS microscopy (Voie et al. 1993). OPFOS was initially developed to image the inner ear cochlea, but it has also been used in ME studies (Voie 2002; Buytaert and Dirckx 2007, 2009). In the OPFOS method, parallel optical sections through a macroscopic biomedical specimen are created by means of a thin sheet of laser light, and the fluorescence originating from within the cross section of the light sheet with the tissue is recorded in the direction perpendicular to the plane of the laser light. The light emitted by the specimen originates from auto-fluorescence or from staining the specimen with a fluorescent dye. OPFOS images both bone and soft tissue at the same time and in real time, as no (back-projection) calculations are required. It allows region-of-interest (ROI) imaging and has both a high sectioning and a high in-plane resolution. Hence, perfectly and automatically aligned images of virtual cross sections can be obtained. OPFOS scanning was performed at the Laboratory of BioMedical Physics at the University of Antwerp (www.ua.ac.be/bimef) with a custom-built setup using bi-directional light-sheet illumination (Buytaert and Dirckx 2009; Buytaert 2010).

For OPFOS imaging, an elaborate specimen preparation is needed (Voie 2002; Buytaert and Dirckx 2009), as the technique requires the specimens to be perfectly transparent. Before μCT scanning, all blood was removed from the blood vessels, as coagulated blood cannot be made transparent afterward. After μCT recording, a 10% neutral buffered formalin bath was applied. Next, all calcium was removed using 10% EDTA in water solution combined with microwaves. Because of this decalcification, the OPFOS method has to be performed second after μCT X-ray scanning. Then, the specimens were dehydrated using a slowly graded ethanol series, up till 100%. Next, all tissue was refractive index matched using a slowly graded Spalteholz fluid series, again up till 100%. As a result, the specimens become entirely transparent when submerged in pure Spalteholz fluid. Finally, to obtain stronger fluorescence, the specimens are stained with rhodamine B.

Both soft tissue and bone were made transparent and fluorescent; hence, both tissue types are visualized with the technique. We focused on ROI OPFOS imaging of ME ligaments, tendons, and muscles, while images of the (often larger) bony structures are more easily obtained from μCT. Comparison of high-resolution μCT and OPFOS data allows us to distinguish bone from soft tissue in the OPFOS data. Merging of the two datasets generates the complete ME model with all of its functional components accounted for.

The shape of the TM was obtained from the μCT data. The OPFOS technique is able to visualize this extremely thin tissue when performing ROI imaging on a small part of the membrane, cf. Figure 2. However, to image the membrane full-field with OPFOS, one needs to zoom out and the resolution needed to adequately visualize this thin membrane is lost. Furthermore, the eardrum is prone to preparation artifacts: Because the gerbil specimens went through an extensive procedure of tissue fixation, decalcification, dehydration, and Spalteholz treatment, the extremely thin TM can get deformed. Therefore, the data on eardrum shape are obtained from the CT images, recorded before any specimen processing was applied. X-rays are normally not suited to image soft tissue, especially if it is very thin, like the eardrum. We tried to counter this problem by applying a droplet of physiological fluid through the ear canal on top of the membrane. The medial border of the droplet and eardrum then becomes more easily distinguishable from air in the ME cavity. In this way, the membrane outline will be obtained without deformation and with adequate resolution.

FIG. 2.

2D virtual cross sections delivered by the OPFOS technique. A Tensor tympani tendon reaching down toward malleus. B Incudomallear and incudostapedial articulation. Pixel size 1.5 × 1.5 μm.

Apart from the specimen preparation, the OPFOS method has another disadvantage as it suffers from stripe artifacts. Opaque regions or areas of less transparency locally reduce the intensity of the laser light sectioning sheet, causing shadow lines or stripes in the rest of the image. This is partially countered by simultaneous dual light-sheet illumination in our setup (Buytaert and Dirckx 2009). Measuring and analyzing the OPFOS data is very time-consuming; therefore, only one gerbil ear has been processed. On the other hand, the μCT data of all three gerbils were analyzed.

Visual observations

We performed visual observations of the orientation, location, shape, and suspension of the ossicular chain inside opened ME bullae with an operating microscope (Zeiss, OPMI Sensera S7). When 3D computer data, models and results were obtained from μCT or OPFOS with striking features, they were compared to qualitative observations of the real geometry in opened bullae with the operating microscope to verify their interpretation. These experiences gave us the necessary expertise to confirm the 3D model results and conclusions of the present paper. For instance, after a targeted dissection, we could visually confirm that the posterior incudal ligament in gerbil indeed exists as one whole band instead of two separate structures, as we found in our OPFOS data and model.

3D segmentation and reconstruction

After obtaining several series of object cross sections—one μCT set originating from back-projection calculations and several ROI datasets from direct OPFOS recordings—we identified and segmented the relevant structures in all images. The goal of segmentation is to locate objects boundaries, which in turn allows software to build 3D surface meshes by triangulation.

In our case, segmentation was done manually for thousands of sections using the commercial image segmentation and 3D surface mesh generating software package Amira 5.3 (Visage Imaging). Manual segmentation might seem primitive and time-consuming, but using our morphological expertise, manual segmentation delivers better results than purely automated segmentation based on thresholding of gray scale values. The Amira software package uses the marching cubes algorithm for triangulation. It takes eight neighboring voxel locations at a time (forming an imaginary cube), after which the polygon(s) needed to represent the part of the isosurface that passes through this cube are determined. The individual polygons are finally fused into the intended surface. This leads to subvoxel triangulation that easily manages sharp angles. When smoothing or simplification (reduction of the number of triangles) is used, the program takes the “steepness” of the surface into account: Flat surface parts are more reduced than curved parts.

As final result, we end up with triangulated surface meshes for the μCT and OPFOS datasets. These can be further developed into finite-element volume meshes using Amira or other packages. On the website of the Laboratory of BioMedical Physics group, we suggest some powerful and open-source volume generating software, e.g., PreView.

Merging of CT and OPFOS models

All cross sections in a μCT dataset and therefore all models of ME components originating from it are inherently perfectly aligned within the data stack. The OPFOS datasets were focused on the soft tissue by separate ROI recordings. However, parts of the bone are included in the OPFOS ROI recordings as well. The cross sections within each ROI OPFOS data stack are also perfectly aligned, but the resulting mesh models per stack are unrelated to the other OPFOS datasets (because of different ROI zooming and/or other slicing orientation) and unrelated to the CT dataset and models.

To merge the OPFOS data with the μCT data, the μCT dataset was used as a reference. We did not merge the 2D image cross sections, but the 3D mesh models: All partial bone models from ROI OPFOS were three-dimensionally aligned to corresponding parts of the μCT models using an iterative spatial transformation least-squares minimization process of the Amira software package. This process uses the iterative closest point (ICP) algorithm to minimize the difference between two point clouds (e.g., all surface nodes of, respectively, an OPFOS and a μCT mesh model). ICP iteratively revises the spatial transformation (6 degrees of freedom for translation and rotation) needed to minimize the Euclidean distance between the points of two datasets. This concept is referred to as the Procrustes superimposition method: The root mean square (RMS) of the distances between corresponding points of the two surfaces is evaluated. Corresponding point pairs are created by finding the closest point of the reference (μCT) surface mesh for each point of the other (OPFOS) surface mesh. When the two surfaces are identical and perfectly superimposed, the RMS of all corresponding point distances will be zero. In the case of the OPFOS versus the μCT stapes model for instance, we obtained a root mean square difference of 17 μm (or two μCT voxels). After obtaining such a good match between the OPFOS and μCT bone model, we applied the same spatial transformation to the OPFOS soft tissue mesh(es) from that OPFOS dataset. In this way, all OPFOS datasets were combined with μCT data into one model.

Results

Computed tomography

Three gerbil ears were recorded with μCT, delivering three isometric data stacks of reconstructed cross sections (pixel size 8.5 × 8.5 μm, separated 8.5 μm). To illustrate the image quality, we present one μCT cross section in Figure 3. Full movies of all cross sections are available on our website, and the entire dataset is available upon request. Notice how distinguishable the ossicle boundaries, the incudomallear and incudostapedial joint cleft, and the annular ligament cleft are in the figure. This high contrast and resolution facilitates the segmentation process considerably.

FIG. 3.

Reconstructed μCT cross section through gerbil 1 (originally 1,496 × 1,496 pixels cropped to 740 × 950 pixels). a middle ear air cavity, c inner ear cochlea, i incus, m malleus, o outer ear canal, s stapes, t tympanic membrane outline. Pixel size is 8.5 × 8.5 μm.

Our main attention went to the ME, but separate 3D surface meshes were also created of the fluid-filled bony labyrinth of the inner ear (cochlea scalae and modiolus, and vestibular apparatus), cf. Figures 1 and 4. The ME bulla air cavities of all gerbils are modeled as well. They give an indication of the enclosed air volume in the ME, cf. Table 1. These segmented volumes include the volume of the ossicles, ligaments, and muscles. Finally, a separate rudimentary mesh of all bone using a fixed segmentation threshold was made. Using transparent rendering for this large model, one can virtually look inside the bulla and observe the ossicles and inner ear inside, cf. Figure 1. We listed volume, dimensions, and several other properties of the ossicles, the TM and the ME bulla cavity in Tables 1, 2, and 3. These and other quantitative data are readily and accurately available from our models.

FIG. 4.

3D surface meshes. Voxel size is 8.5 × 8.5 × 8.5 μm. A Tympanic membrane + middle ear ossicles + inner ear fluid (gerbil 1). B Tympanic membrane + middle ear ossicles (gerbil 2). C Tympanic membrane + middle ear ossicles + inner ear fluid (gerbil 3).

TABLE 1.

Volume, surface area, and number of triangles for G1, G2, and G3 ear components, derived from the 3D surface meshes obtained from μCT

	Volume (mm3)	Area (mm2)	Triangles (#)
Malleus G1	0.670	9.25	384,346
Malleus G2	0.655	9.43	422,420
Malleus G3	0.656	9.16	380,570
Incus G1	0.374	3.85	160,772
Incus G2	0.373	3.85	172,254
Incus G3	0.344	3.54	148,328
Stapes G1	0.085	3.88	159,118
Stapes G2	0.082	3.88	175,242
Stapes G3	0.082	3.65	149,374
Middle ear cavity G1	221.680	386.50	4,999,382
Middle ear cavity G2	238.158	419.69	2,478,224
Middle ear cavity G3	239.542	412.96	2,999,806
Inner ear cavity G1	10.378	65.90	2,729,618
Inner ear cavity G2	10.439	69.14	2,998,432
Inner ear cavity G3	10.789	64.00	2,654,126

The ME cavity volume incorporates the air, ossicles, and ME soft tissue volume

G1 gerbil 1, G2 gerbil 2, G3 gerbil 3

TABLE 2.

Geometry parameters of the TM for G1, G2, and G3 ear components, derived from the 3D surface meshes obtained from μCT

	TM G1	TM G2	TM G3
Diameter tympanic ring inferior–superior (mm) (parallel to the direction of the manubrium)	4.114	4.144	4.168
Diameter tympanic ring anterior–posterior (mm) (orthogonal to the direction of manubrium)	4.190	4.051	4.169
Average diameter of the pars flaccida ring (mm)	1.492	1.335	1.413
Angle between manubrium and plane of tympanic ring (°)	22.6	21.4	23.4
Distance between umbo and plane of tympanic ring (mm)	0.765	0.760	0.769
Surface area pars tensa (mm2)	17.96	17.50	17.35
Surface area pars flaccida (mm2)	2.46	2.24	2.11
Projected surface area pars tensa onto the plane of tympanic ring (mm2)	13.69	13.64	13.22
Projected surface area pars flaccida onto the plane of tympanic ring (mm2)	1.42	1.45	1.54
Thickness near annulus (mm)a	±0.035
Minimal thickness between annulus and umbo (mm)a	±0.007
Thickness near umbo (mm)a	±0.020

G1 gerbil 1, G2 gerbil 2, G3 gerbil 3

^aMean thickness data from confocal microscopy on 11 gerbils by Kuypers et al. (2005)

TABLE 3.

3D length of the manubrium (umbo tip till lateral process tip, cf. Fig. 5) and 3D height of the stapes (medial footplate till tip stapes head) for G1, G2, and G3, derived from the 3D surface meshes obtained from μCT

	Distance (mm)
Manubrium G1	2.747
Manubrium G2	2.704
Manubrium G3	2.729
Stapes footplate–head G1	1.277
Stapes footplate–head G2	1.264
Stapes footplate–head G3	1.273

G1 gerbil 1, G2 gerbil 2, G3 gerbil 3

The mass of malleus, incus, and stapes are, respectively, 1.145, 0.633, and 0.116 mg as reported by Nummela (1995). Adopting these representative values for our specimen in combination with the volumes given in Table 1, we get an average ossicle bone density of 1.37 × 10³ kg/m³ for the stapes and 1.74 × 10³ kg/m³ for incus and malleus.

Note that the outline of the TM was surprisingly but successfully visualized using μCT. The resolution was just high enough to show the shape outline of the extremely thin membrane. Thickness information could not be obtained. Using a fluid droplet in the ear canal to aid in distinguishing the medial border of the eardrum partially failed, as can be seen in Figure 3, fluid is not covering the entire membrane surface in the ear canal because of an air bubble.

Finally, we could observe channels (blood vessels) inside the ossicles, occurring especially in the incus and malleus bone, cf. Figure 5. The ossicular surface shapes are almost identical between all three animals, and the same is true for the size, volume, and branching layout of the major channels inside them.

FIG. 5.

Mesh of the malleus (gerbil 2) rendered transparent in combination with a mesh of the (major) blood vessel channels running inside it. Data obtained from μCT. Voxel size is 8.5 × 8.5 × 8.5 μm.

OPFOS tomography

We will now discuss all identified (soft) tissue structures of the ME of gerbil 2, measured with OPFOS.

Posterior incudal ligament

Using μCT, the posterior incudal ligament cannot be found, cf. Figure 6A, B, while using OPFOS it is clearly visible, cf. Figure 6C–E. This comparison between the two tomographic imaging techniques clearly demonstrates the usefulness of combining the two methods.

FIG. 6.

A μCT cross section and B 3D μCT reconstruction from automatic thresholding do not show the posterior incudal ligament in the bony wall recess. Arrows indicate the position of the invisible ligament. Pixel (and voxel) size is 8.5 × 8.5( × 8.5) μm. C–E ROI OPFOS cross sections from different orientations do show the ligament in the recess. F, G 3D OPFOS meshes. Voxel size is 0.97 × 0.97 × 2.5 μm. b bulla, I incus, L ligament, m malleus.

After segmentation and 3D representation, cf. Figure 6F, G, one can see that the ligament is built as one whole part and forms one sickle-shaped band of fibrous tissue. Its tiny volume amounts to 0.013 mm³. The sickle has its smallest thickness (orthogonally to the image plane of Fig. 6F) of 42 μm near the incus short process and broadens to 190 μm toward the bulla edge. The contact area at the middle ear cavity wall is also a bit larger than the contact area on the incus crus.

Anterior mallear ligament

The anterior process of the malleus has the shape of a (partially opened) handheld Japanese folding fan, reaching toward the anterior bulla wall, cf. Figures 7 and 8. The connective soft tissue of the anterior mallear ligament, which should connect the process to the bulla, is undistinguishable from bone, both in the OPFOS and in the CT recordings. This ligament is probably more ossified or cartilaginous than in some other species, and no separate soft tissue model could be made.

FIG. 7.

Two views of the topography of the chorda tympani in combination with the malleus and the tensor tympani muscle and tendon (gerbil 2). The soft tissue data originate from OPFOS (voxel size is 2 × 2 × 4.5 μm), while the malleus data come from μCT (voxel size is 8.5 × 8.5 × 8.5 μm). b bulla, c chorda tympani, m muscle, o malleus ossicle, t tendon, l manubrium length.

FIG. 8.

OPFOS cross sections showing the course of the chorda tympani with respect to the malleus. A Chorda tympani jumps from a bony support beam to the malleus neck superior side. B It rounds the malleus neck below the tensor tympani. C–E It continues on the anterior process sheet until it enters a fissure in the bulla wall. b bulla, c chorda tympani, o malleus ossicle, t tendon. All subfigures are of the same scale.

Superior mallear and incudal ligament and lateral mallear ligament

According to real-time OPFOS observations, no superior mallear and incudal ligament are present in the gerbil ME, which is confirmed by visual observations with the operating microscope. In addition, no lateral mallear ligament could be discerned with either method.

Tensor tympani muscle and tendon

Figure 2A shows a high-resolution OPFOS section image through the tensor tympani muscle and tendon, the TM, the malleus’ manubrium, and the bulla. This image demonstrates OPFOS’ capability to image bone and soft tissue in high resolution.

After the segmentation and triangulation process, the volume of the tensor tympani muscle and tendon can be calculated from the obtained 3D model and was found to be 0.486 mm³. The distance between the two most distant points on the combined structure is 3.25 mm. The diameter of the muscle tendon varies between 50 and 80 μm.

The cross-sectional area of a muscle (rather than volume or length) determines the amount of force it can generate. A first rough estimate of the order of magnitude of the maximum generated force of the muscle can be derived as follows: By dividing the muscle belly volume by an average muscle fiber length of 400 μm (estimated from the OPFOS images), we end up with a cross-sectional area of 1.2 × 10⁻² cm². A common conversion factor from this area to the maximal isometric contraction force is given by 25 N/cm² for skeletal muscle (Nigg and Herzog 1999), giving a maximally generated force of this muscle of 0.3 N. An interesting comparison of the effect of this force on the malleus can be made by translating this number into a corresponding static pressure working on the TM from the ear canal side. Dividing the force of 0.3 N by the (projected) area of the pars tensa of the TM of gerbil 2 (13.64 mm², cf. Table 2), we obtain a (maximum) static pressure of 22 kPa. The magnitude of this pressure falls in the range of static pressures associated with scuba diving or taking an airplane.

The final merged 3D model shows that the tensor tympani muscle belly is larger than expected from visual observations. Its main part is hidden as it is situated in a gap between the spiraled cochlear dome and the bulla wall.

Stapedial artery

A typical anatomical feature of the gerbil ME is the stapedial artery running through a bony channel on the surface of the first cochlear turn and passing through the stapes crura in the ME air cavity. Using OPFOS, it was possible to image this relatively large stapedial artery, cf. Figure 9. We could even distinguish and separately model the stapedial artery soft tissue wall (the actual blood vessel) and its fluid-filled lumen.

FIG. 9.

Stapes bone, stapedius muscle and tendon, and stapedial artery models obtained from OPFOS (voxel size is 1.5 × 1.5 × 5 μm) and the fluid-filled cavity of the horizontal semi-circular canal from μCT (voxel size is 8.5 × 8.5 × 8.5 μm) are shown (gerbil 2). a artery, m muscle, o stapes ossicle, s semi-circular canal, t tendon, w artery wall.

The diameter of the blood vessel was the smallest in between the crura and amounted to 355 μm with (i.e., outer diameter) and 275 μm without (i.e., inner diameter) the blood vessel soft tissue wall. The wall had a thickness of about 40 μm.

Stapedius muscle and tendon

After segmentation of the stapedius muscle and tendon, we end up with the mesh shown in Figure 9. The tiny volume enclosed in this (tendon and muscle) mesh amounts to 0.085 mm³, and the two most distant points on the combined structure are 1.81 mm apart. The diameter of the tendon varies between 40 and 55 μm. If we again divide the volume by an estimated average muscle fiber length of 350 μm, we get a cross-sectional area of 2.4 × 10⁻³ cm². Multiplying this value by 25 N/cm² gives an estimation of 0.06 N for the maximum force the muscle can produce.

The merged 3D model shows that the stapedius muscle body is attached to the lateral (horizontal) semi-circular canal, cf. Figure 9. In the figure, a gap is seen between the semi-circular canal and the muscle because only the fluid-filled cavity of the canal is shown. When showing bone as well, one sees the muscle clasps firmly around the lateral semi-circular canal wall.

Joint clefts

As can be seen in Figure 2B, the incudomallear and incudostapedial joints can be easily distinguished on high-resolution OPFOS cross sections and appear to form a tight connection. μCT data also show both clefts, from which we made 3D meshes.

The incudomallear joint connects the incus and malleus and has the shape of a twisted saddle. The gap or cleft between the ossicles could contain synovial fluid as it is considered a synovial joint; however, this is not confirmed from our OPFOS measurements nor μCT data in gerbil. No fluid or open space is detected in the joint cleft, and the joint seems quite rigid. This rigidness was already reported for other species by Guinan and Peake (1967) and Gundersen and Høgmoen (1976). The thickness of the joint varies from nearly 0 to 51 μm. The gap or joint tissue is thinner at the lateral side.

The incudostapedial joint connects the incus lenticular process with the head of the stapes. Our model of this synovial joint shows an oval disk with an approximately even thickness of 25.5 μm. Again, the joint cleft seems to possess no synovial fluid and forms a rigid connection, which has also been reported in cat (Funnell et al. 2005).

OPFOS also visualized the stapedial annular ligament cleft in which the annular stapedial ligament is situated, forming a syndesmosis joint. A syndesmosis is a slightly movable articulation where bony surfaces are tightly united by a fibrous tissue ligament (Laurent 1998). The high resolution of the OPFOS data allows to make a 3D mesh of this thin structure, cf. Figure 10. The thickness of the ligament varies between 8 and 18 μm, confirmed by the gap seen in the μCT cleft model which is about 12–18 μm.

FIG. 10.

A–E OPFOS-based models of the stapes and the stapedial annular ligament (gerbil 2). F μCT-based model of the stapes (gerbil 2). The footplate modeled from μCT data is convex, while in the OPFOS model it is not. a annular ligament, c cochlea, o stapes ossicle. The arrow indicates the end of the OPFOS dataset.

Chorda tympani

The chorda tympani nerve branches from the facial nerve and runs through the ME air cavity. In gerbil, the nerve jumps from a sort of support beam at the superior bulla wall to the malleus where it is tightly connected to the malleus neck in the vicinity of which the tensor tympani muscle connects as well, cf. Figure 7. It hangs in the ME air space passing the incudal long process laterally and the manubrium medially. It rounds the malleus neck from the posterior to the anterior side, passing the tensor tympani tendon inferiorly. At the anterior side, it lies on the anterior process sheet until it disappears in a fissure of the bulla wall again. It was unexpected that the chorda tympani could be visualized so well in OPFOS cross sections, cf. Figure 8, because myelin nerve sheets can in principle not be made transparent by the Spalteholz process. Apparently, because the nerve is thin enough, the blurring effect of the less transparent chorda tympani was negligible.

Merging of CT and OPFOS models

As described before, we obtained a series of cross-sectional images from μCT with bone only, cf. Figure 3, and from OPFOS with bony and soft tissue structures, cf. Figures 2, 6, and 8. With OPFOS, we performed ROI recording of all soft tissue structures, so only incomplete parts of the ossicles were measured. However, using these partial models of ossicles and/or bulla bone that were recorded together with the soft tissue, we could align these bony structures (and thus the soft tissue structures as well) to the μCT bone models, cf. Figure 11, using the Procrustes superimposition method.

FIG. 11.

Merged OPFOS-CT ME model (gerbil 2).

The merging and alignment of bony structures revealed that some shrinking of the gerbil 2 specimen had occurred despite of our careful efforts during preparation. Using the warping procedure in Amira (similar to the Procrustes superimposition method, only allowing for scaling in every dimension as well), we found a shrinking factor of 8.4% in all three dimensions. After applying the spatial transformation and upscaling, the OPFOS soft tissue meshes fit rather well in between the CT bone mesh models. For instance, corresponding bony parts of the malleus from OPFOS using a scale factor of 8.4% were aligned with the malleus from μCT. After applying the same scaling and spatial transformation to the tensor tympani, its tendon attaches to the malleus, cf. Figure 11, and at the other side, its muscle body inserts nicely in a bony cavity of the bulla of the inverse shape, cf. Figure 12. This and similar facts give us confidence in the merging of the data.

FIG. 12.

Cross sections at different depths through the 3D merged models of the bulla bone (white) from μCT and the tensor tympani (blue) from OPFOS. Black represents air-filled space such as the ME air cavity. The tensor tympani fits nicely in the bone, rather touching the cavity wall than overlapping with it.

Discussion

Imaging method

Several methods exist to measure and image the ME for the creation of FEM models. μCT in itself is mainly suited to image the bony structures. μCT using contrast agents is a valuable alternative to our combined approach (Metscher 2009). However, it is difficult to discriminate between bone and soft tissue, so it would be necessary to do μCT scans before and after staining and merge the data as we now did with OPFOS. OPFOS offers a resolution down to 2 μm, which is seldom achieved in μCT. For this reason, we preferred OPFOS to obtain the soft tissue data. Multiple energy CT techniques have also proven to be a valuable method for discriminating between soft tissue and bone in CT images (Johnson et al. 2007; Granton et al. 2008). For large macroscopic structures, the technique is indeed feasible; however, it becomes more difficult in the case of microscopic samples. The position of the micro-focus spot changes in an X-ray tube when its energy or source is altered. As a result, the datasets are slightly shifted in a complicated way, and tissue discrimination can no longer be done by simple subtraction or division. Gradually, these technical issues are being solved, so in the future dual-energy CT may be used to measure and discriminate soft tissue and bone.

The most used alternative to our method is conventional histological sectioning, which is unsurpassed in resolution and produces data on the bone and soft tissue simultaneously. Both the histological method and our combined method need a similar specimen preparation that can induce shrinking (Lane and Ráliš 1983; Henson et al. 1994). Our method is considered non-destructive (as multiple measurements can be done on the sample) while the histological method can only measure the sample once and in one slicing orientation because of the need for physical cutting of the specimen. Furthermore, these 2D slices are often deformed during slicing, requiring difficult image processing and registration of all slices before generating a 3D model. μCT and OPFOS each deliver perfectly and automatically aligned cross sections that require no post-processing. Instead of registering every 2D slice, our method only needs to register complete 3D meshes of all submodels to one another. OPFOS further allows real-time virtual sectioning and imaging.

The OPFOS method is one of the the first techniques in a growing field of (laser) light-sheet, now known as the (laser) light-sheet-based fluorescence microscopy (LSFM). The many different implementations and improvements of the technique have been listed in a review article by Buytaert et al. (2011). The construction of an OPFOS/LSFM setup is well feasible in the sense that all parts needed are readily available on the market. Researchers interested in the construction of such a setup or in the collaboration are welcome to contact the authors, and even the first commercial devices are becoming available (Buytaert et al. 2011).

Human versus gerbil

When using animal models, it is important to be aware of the differences with human ME morphology. Figure 13 shows a schematic representation of all human ME components. In addition to the data prepared in this paper, we confirmed our findings in other gerbil ears during other studies using OPFOS and visual inspection with the operation microscope.

FIG. 13.

General schematic overview of all relevant middle ear components in human.

We found that in gerbil, no superior incudal, no superior mallear, and no lateral mallear ligament are present, contrary to the case in humans. The presence and/or function of superior attachments to malleus and incus as suspensory structures is of controversy, though many mathematical models or drawings of the human ME include such structures, cf. Table 2.1 in Mikhael (2005) and Merchant and Nadol (2010).

It has been proposed by Rosowski et al. (1999) that the anterior mallear ligament is a bony connection to the bulla, while Elkhouri et al. (2006) observed the presence of some connective tissue. Our OPFOS measurements could not distinguish any soft tissue, and our CT measurement showed an ossified or cartilaginous connection. The anterior process also had a less pronounced shape in human than the Japanese fan-shaped structure in gerbil.

The posterior incudal ligament, which connects the incus short crus to the fossa incudis, exists in many different configurations, as is illustrated in Figure 14 by Funnell (1972) (based on work by Kobayashi). From the OPFOS sections, cf. Figure 6C–E, the gerbil posterior ligament appears to fall in the category of human and cat configurations. However, it is only possible to appreciate the true configuration in 3D, cf. Figure 6F, G, which clearly places this gerbil ligament in the category of guinea pig and rabbit. The posterior incudal ligament consists of one sickle-shaped part. According to Sim and Puria (2008), it has been observed that in human, the two parts shown in Figure 14 are also connected around the tip of the short crus of the incus to form a single continuous ligament rather than two separate ligaments. In this respect, gerbil and human ME then would be alike.

FIG. 14.

Schematic representation of different posterior incudal ligament configurations per species (courtesy of Funnell 1972). Gerbil falls in the category of guinea pig and rabbit.

We also found the chorda tympani nerve to be present in a special arrangement in gerbil and more tightly connected to the malleus ossicle than in human: In human, this nerve traverses the open space of the ME cavity without actually attaching to the ossicles. In gerbil, there exists a tight connection with the malleus neck and the nerve lies on top of the Japanese fan-shaped anterior process sheet, cf. Figures 7 and 8. Furthermore, the topographic relation of the chorda tympani to the tensor tympani muscle differs from human. In gerbil, it runs hypotensoric (inferiorly to the tensor tympani) and in between the muscle and manubrium, as was confirmed in a recent publication by Ruf et al. (2009), while in human it passes epitensoric (superiorly to the tensor tympani), e.g., Maier (2008).

We derived the ossicle bone density from our volume measurements and from mass data from the literature. We obtained an ossicle bone density of 1.37 × 10³ kg/m³ for the stapes and 1.74 × 10³ kg/m³ for incus and malleus. In comparison to human, the averaged malleus density is found to be 2.31 × 10³ kg/m³, and the averaged incus density is 2.14 × 10³ kg/m³ (Sim and Puria 2008). Another source mentions an average stapes density of 2.2 × 10³ kg/m³ in human (Kirikae 1960; Gan et al. 2004). Hence, gerbil ossicle densities appear to be significantly lower than in human.

Another contrast to human is that the stapedial artery is usually present in gerbil, while seldom in human. Finally, our observations show that the gerbil manubrium of malleus is tightly fused over its full length with the TM, while in human it is mainly only fixed at the tip and lateral process of the manubrium (Koike et al. 2002).

Resolution

We used state-of-the-art X-ray micro-computed tomography and the relatively new orthogonal-plane fluorescence optical-sectioning microscopy on the ME. In previous CT-based studies of the ME, the following model resolutions were reported: 5.5 μm on gerbil (Elkhouri et al. 2006), 6 μm on human (Hagr et al. 2004), 10 μm on cat (Decraemer et al. 2003), and 10 μm on human (Vogel 1999). Though these numbers are comparable to our isometric 8.5-μm voxel size for μCT on gerbil bone, our data and models are of much higher quality than those shown in previous work. One reason might be that the previous authors stated voxel size instead of resolution, while we actually achieve a true resolution of 8.5 μm. Other factors such as scan parameter settings could also account for differences in image quality.

ME soft tissue imaged with medical CT devices gave poor resolution (Lemmerling et al. 1997), and μCT delivered modest resolution (Sim and Puria 2008). The same goes for MRI measurements of gerbil soft tissue structures, e.g., 45 μm (Elkhouri et al. 2006). OPFOS is clearly better suited to achieve high-resolution sections on ligaments and muscles—with pixel sizes ranging from 1 to 5 μm—as can be seen from our sections and 3D models, cf. Figures 2, 6, 7, 8, 9, 10, and 11.

Artifacts

Segmentation of the fluid-filled inner ear channels in the μCT data showed that the round window in all three models is prominently bulged inward toward the cochlea. This might indicate either a small overpressure in the ME air cavity or a loss of cochlear fluid because of dehydration or leakage.

Merging of OPFOS and μCT data revealed shrinking of the soft and bony tissue, most likely caused by the elaborate OPFOS specimen preparation (e.g., tissue fixation, decalcification, dehydration, and Spalteholz treatment), though previous authors reported that this procedure induced negligible shrinking (Voie 2002; Valk et al. 2005; Hofman et al. 2008). Thanks to the combination of OPFOS with μCT, we have undeformed reference data that we can use to derive a scaling factor. Homogeneous scaling with 8% of the OPFOS (bone and soft tissue) models has partially corrected for the shrinking artifact. After decalcification of the sample, bone is reduced to a collagen matrix. The effect of decalcification cannot be investigated with μCT as all calcium is removed and X-ray absorption becomes negligible. It is, however, a reasonable assumption that dehydration will have a similar (and homogeneous) shrinking effect on both soft tissue and decalcified bone. In histology, the same specimen preparation (decalcification and dehydration) is performed, and the same assumption (homogeneous shrinkage) is adopted.

Another artifact related to specimen preparation was noticed on the stapes. The footplate of the stapes is clearly convex and bulges inward to the cochlea in the μCT models, e.g., Figure 10. After decalcification, dehydration, and Spalteholz treatment, the footplate of the OPFOS model showed some relaxation and shriveling of its convex shape. The models available for download therefore consist of μCT data for bone and OPFOS data for soft tissue meshes.

Stripe artifacts in OPFOS were strongly reduced but not entirely eliminated by our bi-directional illumination/sectioning OPFOS setup. In combination with manual segmentation, which also partially corrects for this artifact, no effect remained in the models so no image post-processing of the data was required.

OPFOS was not suited to image the TM, but the full-field outline of the TM shape was obtained from μCT: We could not measure a volume model of the TM with the correct thickness, but only a surface model. FEM modelers can, however, use the surface shape directly as a shell model, cf. Gan et al. (2004) and Elkhouri et al. (2006), and apply either a uniform or a varying measured thickness distribution to their own choosing (as different approaches are taken by different modelers). Table 2 mentions average thickness data at three TM regions, measured on 11 gerbil TMs with confocal microscopy (Kuypers et al. 2005).

Open-source availability

All 3D data and surface mesh models presented in this paper are freely available for educational and research purposes on the website of the Laboratory of BioMedical Physics (http://www.ua.ac.be/bimef/models).

Several educational and research 3D models have also been made available in the past (“3D Virtual Models of the Human Temporal Bone and Related Structures” of Eaton-Peabody Laboratory of Auditory Physiology, http://research.meei.harvard.edu/Otopathology/3dmodels/download.html; “3D Overview of Ear Anatomy” of Ear & Auditory Research Laboratory, http://ear-lab.medicine.dal.ca/3D/3D-Overview.htm; “3D Ear Human Ear” of Auditory Mechanics Laboratory, http://audilab.bmed.mcgill.ca/∼daren/3Dear/; “The Vertebrate Ear and Temporal Bone” of Auditory Research Laboratory, http://cbaweb2.med.unc.edu/henson_mrm/; “MicroCT Data and 3D Reconstructions” of OtoBiomechanics Group, http://www.stanford.edu/∼puria1/Site/Imaging.html).

Conclusion

Finite-element computer modeling needs accurate 3D models to obtain realistic simulation results for middle ear mechanics. 3D models are also useful in medical training or for the interpretation and presentation of experimental results. The middle ear does not only comprise the ossicles but also consists of soft tissue: tympanic membrane, ligaments, muscles, tendon, and blood vessels.

In this paper, we presented an accurate and complete morphological 3D middle (and inner) ear model of gerbil. The model is freely available to the research community at our website. The presented model quality is unprecedented. The position, orientation, and size of all components making up the gerbil middle ear are now accurately known and individually discussed.