Fig. 2.

Rg1 does not affect HIF-1α protein degradation but increases its protein synthesis. a HUVECs treated with Rg1 (150 nM) for 0, 1, and 3 h. Total RNA was isolated, and RT-PCR was performed using HIF-1α and HIF-1β sequence-specific primers. GAPDH was included as the internal control. b Cells treated with Rg1 were incubated in the presence of cycloheximide (CHX, 5 μg/ml) for 60 min and then subjected to immunoblotting with anti-HIF-1α. β-actin was used as a loading control. The signal intensities were determined by densitometry. Data are shown as mean ± SD of three independent experiments. *P < 0.05, difference with untreated control