. Author manuscript; available in PMC: 2012 Jul 26.

Published in final edited form as: Biochemistry. 2011 Jun 30;50(29):6301–6307. doi: 10.1021/bi200878c

Table 2.

Parameters for PABA to thrombin wild type and mutants N143P and Y225P.

Enzyme	F₀ (V)^a	F₁ (V)^a	ΔF/F₀ (%)^a	K_app (mM⁻¹)^a	K_A (mM⁻¹)^b	k_r (s⁻¹)^b	k_−r (s⁻¹)^b	r^b
wt	6.66±0.04	9.70±0.03	46	17±1	40±2	91±2	120±10	1.3±0.1
N143P	8.25±0.05	9.20±0.05	12	4.0±0.3	10±1	200±10	290±10	1.5±0.2
Y225P	5.96±0.03	7.20±0.04	21	7.6±0.5	28±2	170±10	460±20	2.7±0.3

From the data in Figure 3A–C using eq 1 in the text.

From the data in Figure 2D using eq 3 in the text. The values of k_r and k_−r derived for wild-type from rapid kinetics of Na⁺ binding are 115±3 s⁻¹ and 83±6 s⁻¹ (20).