Correction
A confocal image in Figure 3B of Hauser et al. 2010 [1], showing TGN staining of Vphu-HcRed expressing cells (middle row,) was incorrect. This image has now been replaced with the correct image.
Supplementary Material
Updated versions of Figure 3 of Hauser et al. 2010 [1]. Redistribution of tetherin to an intracellular compartment by HIV anti-tetherin factors. (A) The percentage of HeLa cells displaying tetherin concentrated in a perinuclear compartment (PNC) was calculated for 100 cells, from either control (Ctrl.) cells or cells transfected with 2 μg of Vpu or ROD10 Env expression plasmids. Mean +/- SEM is shown for n = 2 independent experiments. (B) HeLa cells transfected with either Vpu (Vphu-HcRed) or ROD10 Env, showed increased concentration of tetherin in a perinuclear compartment (arrowed), that co-stained with the TGN marker, TGN46. The triple color merged image is shown. Scale bars represent 10 μM.
Contributor Information
Heiko Hauser, Email: Hauser.heiko@gmail.com.
Lisa A Lopez, Email: Lisa.lopez@usc.edu.
Su Jung Yang, Email: suyang@usc.edu.
Jill E Oldenburg, Email: jhenley@usc.edu.
Colin M Exline, Email: cexline@usc.edu.
John C Guatelli, Email: jguatelli@ucsd.edu.
Paula M Cannon, Email: pcannon@usc.edu.
References
- Hauser Heiko, Lopez Lisa A, Yang Su J, Oldenburg Jill E, Exline Colin M, Guatelli John C, Cannon Paula M. HIV-1 Vpu and HIV-2 Env counteract BST-2/tetherin by sequestration in a perinuclear compartment. Retrovirology. 2010;7:51. doi: 10.1186/1742-4690-7-51. [DOI] [PMC free article] [PubMed] [Google Scholar]
Associated Data
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Supplementary Materials
Updated versions of Figure 3 of Hauser et al. 2010 [1]. Redistribution of tetherin to an intracellular compartment by HIV anti-tetherin factors. (A) The percentage of HeLa cells displaying tetherin concentrated in a perinuclear compartment (PNC) was calculated for 100 cells, from either control (Ctrl.) cells or cells transfected with 2 μg of Vpu or ROD10 Env expression plasmids. Mean +/- SEM is shown for n = 2 independent experiments. (B) HeLa cells transfected with either Vpu (Vphu-HcRed) or ROD10 Env, showed increased concentration of tetherin in a perinuclear compartment (arrowed), that co-stained with the TGN marker, TGN46. The triple color merged image is shown. Scale bars represent 10 μM.