FIG 3 .

Growth kinetics of V. fischeri strains with hemin as an iron source. Cultures of the wild type (circles), AKD910 (heme uptake mutant; triangles), and the ΔyfeX mutant (squares) were grown overnight in LBS medium and diluted 1:1,000 in mineral salts medium with 50 µM bipyridyl with (filled symbols) or without (open symbols) hemin (50 µg/ml) supplementation. Two hundred-microliter aliquots of the diluted cultures were incubated in a 96-well plate at 28°C, and cell density was measured at 595 nm every 30 min using a Bio-Tek Synergy 2 plate reader. Error bars show standard errors.