FIG 4 .
Impact of YfeX on porphyrin accumulation during growth of E. coli and V. fischeri. E. coli strains containing plasmid constructs as described in the Materials and Methods were grown on LB plates with or without 50 µg/ml hemin or 50 µg/ml ALA. V. fischeri was grown on LBS plates with 2 mM IPTG with or without 50 µg/ml hemin and 100 µM bipyridyl or 50 µg/ml ALA. Plates were incubated at 37°C (E. coli) and 28°C (V. fischeri) for 3 days and imaged using bright-field microscopy (F6.6, 1/60-s exposure) (left columns) and a red/green fluorescence filter (F6.6, 1/2-s exposure) (right columns).