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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Biochemistry. 2007 Sep 18;46(40):11389–11397. doi: 10.1021/bi700125e

Table 3.

Effect of the substitutions of unique amino acid residues of hMC2R on 125I ACTH binding and cAMP production

Receptor expression (% of WT) 125I- ACTH binding
Ki (nM)		 cAMP production
EC50 (nM)
hMC2RWT 100 8.6 ± 1.0 4.8 ± 0.1
N81A 89 ± 12.4 15.9 ± 0.6* 11.6 ± 1.2*
D104N 78 ± 13.4 87.3 ± 9.8* 87.7 ± 9.3*
F108A 65 ± 10.2 >103* >103*
T164A 93 ± 14.2 6.4 ± 0.7 3.9 ± 0.1
F168A 56 ± 9.5 217.8 ± 13* 327.6 ± 30.3*
F244A 95 ± 11.2 9.9 ± 0.9 4.9 ± 0.9
*

P<0.05 compared with WT receptor