Table 1.
Analysis of differential gene expression in primary TLR2 KO versus WT microglia in response to intact S. aureus
| Gene Name* | Accession # | Z-score (TLR2 KO vs. TLR2 WT) |
|---|---|---|
| Signaling | ||
| SOX12 | NM_011438.2 | 4.65 |
| MAP2K1 | NM_008927.3 | 4.16 |
| RAB10 | NM_016676.4 | 3.93 |
| Jak1 | NM_146145.2 | 3.89 |
| c-Fos | NM_010234.2 | 3.78 |
| RAB9 | NM_019773.1 | 3.37 |
| REL | NM_009044.2 | 3.125 |
| RIN2 | NM_028724.3 | 3.11 |
| SOX21 | NM_177753.2 | 3.03 |
| Rgs-1 | NM_015811.1 | 2.79 |
| NFIB | NM_008687.2 | 2.65 |
| NFKBIB | NM_010908.3 | 2.64 |
| RhoB | NM_007483.2 | 2.46 |
| MAP4K4 | NM_008696.2 | 2.23 |
| VAV3 | NM_020505.2 | 2.23 |
| RelB | NM_009046.2 | 2.04 |
| ASAH1 | NM_019734.1 | -7.14 |
| UNC93B | NM_019449.1 | -5.66 |
| PIK3AP1 | NM_031376.3 | -4.93 |
| RAB7 | NM_009005.1 | -4.77 |
| PLD3 | NM_011116.1 | -7.77 |
| IRF1 | NM_008390.1 | -4.53 |
| SGK3 | NM_177547.3 | -4.42 |
| IRF9 | NM_008394.2 | -4.07 |
| IRAK3 | NM_028679.3 | -2 |
| Chemokine | ||
| CXCL9 | NM_008599.3 | 5.59 |
| CXCL4 | NM_019932.2 | 3.85 |
| CXCL1 | NM_008176.1 | -4.73 |
| Cytokine | ||
| IFN-β | NM_010510.1 | 1.52 |
| TNFRSF1B | NM_011610.1 | -8.3 |
| G-CSF | NM_009971.1 | -6.93 |
| IL-10 | NM_010548.1 | -4.53 |
| TGF-β1 | NM_009369.3 | -3.53 |
| Receptor | ||
| AIF1 | NM_019467.2 | 3.38 |
| CSF2RB2 | NM_007781.2 | 2.56 |
| IL-10RB | NM_008349.4 | 2.53 |
| TLR2 | NM_011905.2 | -18.7 |
| EMR1 | NM_010130.3 | -7.64 |
| P2RX4 | NM_011026.2 | -4.86 |
| LOX | NM_010728.1 | -4.12 |
| TLR8 | NM_133212.2 | -3.88 |
| Enzymes | ||
| PTGS2 | NM_011198.2 | 4.45 |
| PTGES3 | NM_019766.4 | 4.38 |
| CTSA | NM_001038492.1 | 3.85 |
| CTSS | NM_021281.1 | 3.83 |
| ADAM9 | NM_007404.1 | 3.74 |
| MMP3 | NM_010809.1 | 4.1 |
| CAT | NM_009804.1 | 2.0 |
| CTSZ | NM_022325.4 | 1.7 |
| NOS2 | NM_010927.3 | -6.36 |
| TIMP-1 | NM_011593.2 | -5.55 |
| MMP13 | NM_008607.1 | -4.97 |
| Miscellaneous | ||
| RTN1 | NM_153457.6 | 6.1 |
| VCAM-1 | NM_011693.2 | 2.2 |
MAP2K1, mitogen-activated protein kinase kinase 1; RAB10, Ras-related protein Rab-10; Jak1, Janus kinase 1; RAB9, Ras-related protein Rab-9; RIN2, Ras and Rab interactor 2; Rgs-1, regulator of G-protein signaling 1; NFIB, nuclear factor I/B; NFKBIB, NF-kappa-B inhibitor beta; RhoB, Ras homolog gene family, member B; MAP4K4, mitogen-activated protein kinase kinase kinase kinase 4; VAV3, guanine nucleotide exchange factor VAV3; ASAH1, acid ceramidase; UNC93B, transmembrane endoplasmic reticulum protein UNC-93B; PIK3AP1, Phosphoinositide 3-kinase adapter protein 1; RAB7, Ras-related protein Rab-7; PLD3, phospholipase D3; IRF1, interferon regulatory factor 1; SGK3, serum/glucocorticoid regulated kinase-like; IRF9, interferon regulatory factor 9; IRAK3, interleukin-1 receptor-associated kinase 3; CXCL9, monokine induced by IFN-γ (MIG); CXCL4, platelet factor-4 (PF4); CXCL1, keratinocyte-derived cytokine (KC); IFN-β, interferon-beta; TNFRSF1B, tumor necrosis factor receptor superfamily member 1B; G-CSF, granulocyte-colony stimulating factor; IL-10, interleukin-10; TGF-β1, transforming growth factor-β1; AIF1, allograft inflammatory factor 1; CSF2RB2, colony stimulating factor 2 receptor, beta 2; IL-10RB, interleukin-10 receptor beta; TLR2, Toll-like receptor 2; EMR1, EGF-like module-containing mucin-like hormone receptor-like 1; P2RX4, P2X purinoceptor 4; LOX, lectin-like oxidized LDL receptor; TLR8, Toll-like receptor 8; PTGS2, prostaglandin-endoperoxide synthase 2, cyclooxygenase-2 (COX-2); PTGES3, prostaglandin E synthase 3; CTSA, cathepsin A; CTSS, cathepsin S; ADAM9, disintegrin and metalloproteinase domain-containing protein 9; MMP3, matrix metalloproteinase-3; CAT, catalase; CTSZ, cathepsin Z; NOS2, inducible nitric oxide synthase; TIMP-1, tissue inhibitor of metalloproteinase-1; MMP-13, matrix metalloproteinase-13; RTN1, reticulon-1; VCAM-1, vascular cellular adhesion molecule-1.