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. 1985 Jul 11;13(13):4777–4788. doi: 10.1093/nar/13.13.4777

Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants.

R Deblaere, B Bytebier, H De Greve, F Deboeck, J Schell, M Van Montagu, J Leemans
PMCID: PMC321826  PMID: 4022773

Abstract

A two-component cloning system to transfer foreign DNA into plants was derived from the octopine Ti plasmid pTiB6S3. pGV2260 is a non-oncogenic Ti plasmid from which the T-region is deleted and substituted by pBR322. pGV831 is a streptomycin-resistant pBR325 derivative that contains a kanamycin resistance marker gene for plant cells and a site for cloning foreign genes between the 25-bp border sequences of the octopine T-region. Conjugative transfer of pGV831 derivatives to Agrobacterium and cointegration by homologous recombination between the pBR322 sequences present on pGV831 and pGV2260, can be obtained in a single step. Strains carrying the resulting cointegrated plasmids transfer and integrate T-DNA into the genome of tobacco protoplasts, and transformed tobacco calli are readily selected as resistant to kanamycin. Intact plants containing the entire DNA region between the T-DNA borders have been regenerated from such clones. In view of these properties we present pGV831 and its derivatives as vectors for efficient integration of foreign genes into plants.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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