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. 1985 Aug 26;13(16):5747–5760. doi: 10.1093/nar/13.16.5747

Type II restriction endonucleases cleave single-stranded DNAs in general.

K Nishigaki, Y Kaneko, H Wakuda, Y Husimi, T Tanaka
PMCID: PMC321909  PMID: 2994012

Abstract

Restriction endonucleases (13 out of 18 species used for the test) were certified to cleave single-stranded(ss)DNA. Such enzymes as AvaII, HaeII, DdeI, AluI, Sau3AI, AccII,TthHB8I and HapII were newly reported to cleave ssDNA. A model to account for the cleavage of ssDNA by restriction enzymes was proposed with supportive data. The essential part of the model was that restriction enzymes preferentially cleave transiently formed secondary structures (called canonical structures) in ssDNA composed of two recognition sequences with two fold rotational symmetry. This means that a restriction enzyme can cleave ssDNAs in general so far as the DNAs have the sequences of restriction sites for the enzyme, and that the rate of cleavage depends on the stabilities of canonical structures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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