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. 1985 Aug 26;13(16):5907–5917. doi: 10.1093/nar/13.16.5907

N-acetoxy-2-acetylaminofluorene modification of a deoxyoligonucleotide duplex.

D G Sanford, T R Krugh
PMCID: PMC321921  PMID: 4034398

Abstract

The carcinogen N-acetoxy-2-acetylaminofluorene was reacted with d (CCACGCACC) to form a covalent adduct with attachment at the single guanine. The sample was purified, mixed 1:1 with d (GGTGCGTGG) and studied by thermal denaturation experiments. The Tm for the mixture was 35 +/- 3 degrees C, consistent with duplex formation. The method of continuous variation shows that the modified oligomer, d (CCACGAAFCACC), forms a 1:1 duplex with d (GGTGCGTGG). Circular dichroism spectra also indicate the formation of a duplex and suggest that the modified duplex has a left-handed conformation. Addition of the intercalating drug ethidium alters the CD spectrum of the modified duplex, resulting in a CD spectrum similar to that of ethidium bound to right-handed DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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