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. 1985 Oct 11;13(19):7015–7024. doi: 10.1093/nar/13.19.7015

Interaction of the restriction endonuclease ScaI with its substrates.

K Kita, N Hiraoka, F Kimizuka, A Obayashi, H Kojima, H Takahashi, H Saito
PMCID: PMC322019  PMID: 2997733

Abstract

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.

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Selected References

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