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. 1985 Oct 11;13(19):7119–7128. doi: 10.1093/nar/13.19.7119

Synthesis and hybridization of dodecadeoxyribonucleotides containing a fluorescent pyridopyrimidine deoxynucleoside.

H Inoue, A Imura, E Ohtsuka
PMCID: PMC322027  PMID: 4059054

Abstract

Partially self-complementary dodecadeoxyribonucleotides containing a fluorescent nucleoside, 3-beta-D-2'-deoxyribofuranosyl-2, 7-dioxopyrido[2, 3-d]pyrimidine (pyridopyrimidine deoxynucleoside, dF) were synthesized by the phosphotriester solidphase method. A dodecanucleotide d(GGGAAFGTTCCC) pairing the analog and guanine at the centre of the chain showed a higher melting temperature than the corresponding G-C paired duplex. A similar comparison between A-T and A-F suggested that weaker hydrogen bonds exist when adenine and pyridopyrimidine residues are paired.

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Selected References

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  1. Broka C., Hozumi T., Arentzen R., Itakura K. Simplications in the synthesis of short oligonucleotide blocks. Nucleic Acids Res. 1980 Nov 25;8(22):5461–5471. doi: 10.1093/nar/8.22.5461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ikehara M., Ohtsuka E., Tokunaga T., Taniyama Y., Iwai S., Kitano K., Miyamoto S., Ohgi T., Sakuragawa Y., Fujiyama K. Synthesis of a gene for human growth hormone and its expression in Escherichia coli. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5956–5960. doi: 10.1073/pnas.81.19.5956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jay E., Bambara R., Padmanabhan R., Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. doi: 10.1093/nar/1.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Millican T. A., Mock G. A., Chauncey M. A., Patel T. P., Eaton M. A., Gunning J., Cutbush S. D., Neidle S., Mann J. Synthesis and biophysical studies of short oligodeoxynucleotides with novel modifications: a possible approach to the problem of mixed base oligodeoxynucleotide synthesis. Nucleic Acids Res. 1984 Oct 11;12(19):7435–7453. doi: 10.1093/nar/12.19.7435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ohtsuka E., Matsuki S., Ikehara M., Takahashi Y., Matsubara K. An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. J Biol Chem. 1985 Mar 10;260(5):2605–2608. [PubMed] [Google Scholar]
  6. Ohtsuka E., Nishikawa S., Markham A. F., Tanaka S., Miyake T., Wakabayashi T., Ikehara M., Sugiura M. Joining of 3'-modified oligonucleotides by T4 RNA ligase. Synthesis of a heptadecanucleotide corresponding to the bases 61--77 from Escherichia coli tRNAfMet. Biochemistry. 1978 Nov 14;17(23):4894–4899. doi: 10.1021/bi00616a006. [DOI] [PubMed] [Google Scholar]
  7. Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]

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