Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1985 Oct 25;13(20):7195–7206. doi: 10.1093/nar/13.20.7195

5-Methylcytidylic modification of in vitro transcript from the rat identifier sequence; evidence that the transcript forms a tRNA-like structure.

K Sakamoto, N Okada
PMCID: PMC322038  PMID: 2414735

Abstract

Previous studies showed that the rat identifier sequence, the rodent type 2 Alu family, the rabbit C family and the bovine or goat 73 bp repeat show remarkable resemblance with a few specific tRNA molecules (Sakamoto and Okada, J. Mol. Evol. in press). This paper reports 5-methylcytidylic modification of the in vitro transcript from the ID sequence, and provides further evidence that the transcript forms a tRNA-like structure in vitro. Fingerprint analysis and oligonucleotide mapping suggested that the sequence of the oligonucleotide containing 5-methylcytidine is CpCpm5CpUpGp, which corresponds to the extra and T psi stem regions in the secondary structure of phenylalanine tRNA. Since the sequence of the corresponding oligonucleotide in the phenylalanine tRNA is Cpm5CpCpUpGp, these results suggests that a tRNA (cytosine-5) methyl transferase recognizes the secondary structure of the transcript from the ID sequence rather than the primary sequence. The significance of this modification in relation to the functional role of the ID sequence is discussed.

Full text

PDF
7195

Images in this article

7198Image
on p.7198
7200Image
on p.7200
7201Image
on p.7201

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ames B. N., Tsang T. H., Buck M., Christman M. F. The leader mRNA of the histidine attenuator region resembles tRNAHis: possible general regulatory implications. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5240–5242. doi: 10.1073/pnas.80.17.5240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Lerner M. R., Andrews N. C., Miller G., Steitz J. A. Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1981 Feb;78(2):805–809. doi: 10.1073/pnas.78.2.805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Manley J. L., Fire A., Cano A., Sharp P. A., Gefter M. L. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855–3859. doi: 10.1073/pnas.77.7.3855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Milner R. J., Bloom F. E., Lai C., Lerner R. A., Sutcliffe J. G. Brain-specific genes have identifier sequences in their introns. Proc Natl Acad Sci U S A. 1984 Feb;81(3):713–717. doi: 10.1073/pnas.81.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Okada N., Nishimura S. Isolation and characterization of a guanine insertion enzyme, a specific tRNA transglycosylase, from Escherichia coli. J Biol Chem. 1979 Apr 25;254(8):3061–3066. [PubMed] [Google Scholar]
  7. Okada N., Yasuda T., Nishimura S. Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA. Nucleic Acids Res. 1977 Dec;4(12):4063–4075. doi: 10.1093/nar/4.12.4063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sakamoto K., Kominami R., Mishima Y., Okada N. The 6S RNA transcribed from rodent total DNA in vitro is the transcript of the type 2 Alu family. Mol Gen Genet. 1984;194(1-2):1–6. doi: 10.1007/BF00383489. [DOI] [PubMed] [Google Scholar]
  9. Sutcliffe J. G., Milner R. J., Bloom F. E., Lerner R. A. Common 82-nucleotide sequence unique to brain RNA. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4942–4946. doi: 10.1073/pnas.79.16.4942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sutcliffe J. G., Milner R. J., Gottesfeld J. M., Lerner R. A. Identifier sequences are transcribed specifically in brain. Nature. 1984 Mar 15;308(5956):237–241. doi: 10.1038/308237a0. [DOI] [PubMed] [Google Scholar]
  11. Sutcliffe J. G., Milner R. J., Gottesfeld J. M., Reynolds W. Control of neuronal gene expression. Science. 1984 Sep 21;225(4668):1308–1315. doi: 10.1126/science.6474179. [DOI] [PubMed] [Google Scholar]
  12. Talkington C. A., Nishioka Y., Leder P. In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7132–7136. doi: 10.1073/pnas.77.12.7132. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES