Table 2.

Deconvolution of ΔS spectra of membrane-reconstituted Fgp41^a

Fgp41 labeling	Peak shift (ppm) b		Peak width (ppm) c	Intensity (fraction of total)
1-13C, 15N Leu	182.1	helix	1.4	0.10
	178.3		3.1	0.82
	174.6	β	1.5	0.08
1-13C Phe + 15N Leu	176.9	helix	4.9	0.37
	172.6	β	4.3	0.63
1-13C Val + 15N Phe	181.8	helix	3.3	0.12
	177.4		3.9	0.57
	172.2	β	4.2	0.31

^aSpectral deconvolution was done with two or three Gaussian lineshapes whose peak shifts, linewidths, and intensities were independently varied until there was minimal difference between the sum of the lineshapes and the experimental lineshape. For all cases, there was excellent agreement between the best-fit deconvolution sum lineshape and experimental lineshape, see Supporting Information. Deconvolution was not meaningful for the other samples because the ΔS spectra were broad and relatively featureless.

^bThe reasons for assignment of peaks to specific conformations are provided in the main text.

^cFull-width at half-maximum linewidth.