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. 1985 Nov 11;13(21):7829–7836. doi: 10.1093/nar/13.21.7829

An NMR study of A-T base pair opening rates in oligonucleotides. Influence of sequence and of adenine methylation.

E Quignard, R Téoule, A Guy, G V Fazakerley
PMCID: PMC322089  PMID: 4069998

Abstract

We report relaxation time measurements by semi-selective and totally selective NMR techniques on the thymidine imino protons of d(GGATATCC) and d(GGm6ATATCC). For these oligonucleotides helix fraying, rather than single base pair opening, is the major exchange mechanism even 25 degrees C below the Tm. We have therefore applied a new saturation transfer technique to measure exchange rates at temperatures where fraying has a very small or negligible contribution. Measurements of exchange rates as a function of temperature give significantly different activation energies for base pairs 3 and 4 in d(GGATATCC). Adenine methylation results in a slowing down of the opening rate for the m6A-T base pair but surprisingly has an even greater effect upon the adjacent non-methylated A-T base pair.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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