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. 1985 Dec 9;13(23):8305–8321. doi: 10.1093/nar/13.23.8305

Detection of a subgenomic mRNA for gene V, the putative reverse transcriptase gene of cauliflower mosaic virus.

A L Plant, S N Covey, D Grierson
PMCID: PMC322136  PMID: 2417196

Abstract

Polypeptides synthesized in vitro in rabbit reticulocyte lysates, directed by poly(A)+ RNA isolated from turnip leaves infected with cauliflower mosaic virus (CaMV), were analysed by polyacrylamide gel electrophoresis. Following translation of virus-specific RNA purified by hybrid-selection using CaMV DNA immobilized on DBM papers, the polypeptides observed included the viral gene VI inclusion body protein P62, and a larger product, P75, together with several smaller polypeptides. By translating RNA hybrid-selected with restriction fragments encompassing the CaMV genome, a mRNA for P75 has been mapped to gene V. These results, together with sucrose gradient ultracentrifugation studies, suggest that a CaMV gene V mRNA is a sub-genomic transcript of approximately 2.5Kb and 22S. The expression strategy of the CaMV genome is discussed in the light of our findings.

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Selected References

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