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. 1969 Feb;48(2):229–234. doi: 10.1172/JCI105979

The influence of ouabain and alpha angelica lactone on calcium metabolism of dog cardiac microsomes

Mark L Entman 1,2, Joseph W Cook Jr 1,2, Rubin Bressler 1,2
PMCID: PMC322214  PMID: 4236805

Abstract

The influence of ouabain and alpha angelica lactone on 45calcium accumulation in cardiac microsomes was studied. Calcium binding (accumulation in the absence of excess oxalate or phosphate) was augmented by both ouabain and alpha angelica lactone in the presence of adenosine triphosphate (ATP) but unaffected in its absence. Calcium turnover (defined as the change in 45Ca++ bound to the microsomes after the specific activity is changed) was studied to determine if the augmented bound pool was freely exchangeable at equilibrium. Ouabain and alpha angelica lactone augmented calcium turnover in both the presence and absence of ATP. Calcium-stimulated ATPase was increased by both agents.

It is proposed that these two unsaturated lactones, with known cardiotonic activity, may exert their effects by providing an increased contraction-dependent calcium pool to be released upon systolic depolarization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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