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. 1969 Feb;48(2):271–283. doi: 10.1172/JCI105983

Effect of changes in renal perfusion pressure on the suppression of proximal tubular sodium reabsorption due to saline loading

Norman Bank 1, Karl M Koch 1, Hagop S Aynedjian 1, Marc Aras 1
PMCID: PMC322218  PMID: 5765784

Abstract

Rapid intravenous infusion of saline is known to suppress reabsorption of sodium and water in the proximal tubule. It has previously been shown that this suppression is accompanied by two changes which in combination might account for the over-all decrease in reabsorption: a reduction in the intrinsic reabsorptive capacity of the tubular epithelium (C/πr2) and a reduction in the ratio between tubular volume and GFR (πr2d/Vo). The present micropuncture experiments were carried out in order to study the possible role of altered peritubular physical forces (hydrostatic and colloid oncotic pressure) in mediating these two changes. Proximal tubular reabsorptive capacity, transit time, fractional reabsorption of sodium and water, πr2d/Vo, and intratubular hydrostatic pressure were measured in saline-loaded rats during acute changes in renal perfusion pressure induced by intermittent constriction of the abdominal aorta.

We found that when renal perfusion pressure was lowered to 70-90 mm Hg, the usual effects of saline loading on C/πr2, πr2d/Vo, and fractional reabsorption in the proximal tubule were greatly minimized. When the aortic clamp was released and renal perfusion pressure allowed to rise, C/πr2, πr2d/Vo, and fractional reabsorption fell markedly to levels characteristically seen in saline diuresis. Reclamping of the aorta reversed all of these changes.

In order to determine whether the changes in C/πr2 accompanying changes in renal perfusion pressure were mediated by a circulating natriuretic hormone, we assayed in hydopenic rats the dialysate of plasma collected from saline-loaded rats during and after release of aortic constriction by the split oil drop method. No significant difference in reabsorptive half-time (t½) was found between the two dialysates, and t½ with both dialysates was approximately the same as was found when isotonic saline was injected in the tubules of hydropenic control animals. These observations suggest that the large changes in C/πr2 which occurred with changes in renal perfusion pressure in saline-loaded rats were not mediated by a circulating hormone. We suggest that the reduction in C/πr2, πr2d/Vo, and fractional reabsorption which occurs in the proximal tubule during a saline diuresis is related to the rise in hydrostatic pressure within the kidney.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANK N. Relationship between electrical and hydrogen ion gradients across rat proximal tubule. Am J Physiol. 1962 Sep;203:577–582. doi: 10.1152/ajplegacy.1962.203.3.577. [DOI] [PubMed] [Google Scholar]
  2. BLYTHE W. B., WELT L. G. DISSOCIATION BETWEEN FILTERED LOAD OF SODIUM AND ITS RATE OF EXCRETION IN THE URINE. J Clin Invest. 1963 Sep;42:1491–1496. doi: 10.1172/JCI104833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bank N., Aynedjian H. S. A micropuncture study of renal bicarbonate and chloride reabsorption in hypokalaemic alkalosis. Clin Sci. 1965 Aug;29(1):159–170. [PubMed] [Google Scholar]
  4. Bossert W. H., Schwartz W. B. Relation of pressure and flow to control of sodium reabsorption in the proximal tubule. Am J Physiol. 1967 Sep;213(3):793–802. doi: 10.1152/ajplegacy.1967.213.3.793. [DOI] [PubMed] [Google Scholar]
  5. Brenner B. M., Bennett C. M., Berliner R. W. The relationship between glomerular filtration rate and sodium reabsorption by the proximal tubule of the rat nephron. J Clin Invest. 1968 Jun;47(6):1358–1374. doi: 10.1172/JCI105828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brunner F. P., Rector F. C., Jr, Seldin D. W. Mechanism of glomerulotubular balance. II. Regulation of proximal tubular reabsorption by tubular volume, as studied by stopped-flow microperfusion. J Clin Invest. 1966 Apr;45(4):603–611. doi: 10.1172/JCI105374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cortney M. A., Mylle M., Lassiter W. E., Gottschalk C. W. Renal tubular transport of water, solute, and PAH in rats loaded with isotonic saline. Am J Physiol. 1965 Dec;209(6):1199–1205. doi: 10.1152/ajplegacy.1965.209.6.1199. [DOI] [PubMed] [Google Scholar]
  8. DE WARDENER H. E., MILLS I. H., CLAPHAM W. F., HAYTER C. J. Studies on the efferent mechanism of the sodium diuresis which follows the administration of intravenous saline in the dog. Clin Sci. 1961 Oct;21:249–258. [PubMed] [Google Scholar]
  9. DIRKS J. H., CIRKSENA W. J., BERLINER R. W. THE EFFECTS OF SALINE INFUSION ON SODIUM REABSORPTION BY THE PROXIMAL TUBULE OF THE DOG. J Clin Invest. 1965 Jul;44:1160–1170. doi: 10.1172/JCI105223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. EARLEY L. E., FRIEDLER R. M. CHANGES IN RENAL BLOOD FLOW AND POSSIBLY THE INTRARENAL DISTRIBUTION OF BLOOD DURING THE NATRIURESIS ACCOMPANYING SALINE LOADING IN THE DOG. J Clin Invest. 1965 Jun;44:929–941. doi: 10.1172/JCI105210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. EARLY L. E., FRIEDLER R. M. OBSERVATIONS ON THE MECHANISM OF DECREASED TUBULAR REABSORPTION OF SODIUM AND WATER DURING SALINE LOADING. J Clin Invest. 1964 Oct;43:1928–1937. doi: 10.1172/JCI105067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Earley L. E., Friedler R. M. Studies on the mechanism of natriuresis accompanying increased renal blood flow and its role in the renal response to extracellular volume expansion. J Clin Invest. 1965 Nov;44(11):1857–1865. doi: 10.1172/JCI105293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Earley L. E., Friedler R. M. The effects of combined renal vasodilatation and pressor agents on renal hemodynamics and the tubular reabsorption of sodium. J Clin Invest. 1966 Apr;45(4):542–551. doi: 10.1172/JCI105368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Earley L. E., Martino J. A., Friedler R. M. Factors affecting sodium reabsorption by the proximal tubule as determined during blockade of distal sodium reabsorption. J Clin Invest. 1966 Nov;45(11):1668–1684. doi: 10.1172/JCI105474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GOTTSCHALK C. W., MYLLE M. Micropuncture study of pressures in proximal and distal tubules and peritubular capillaries of the rat kidney during osmotic diuresis. Am J Physiol. 1957 May;189(2):323–328. doi: 10.1152/ajplegacy.1957.189.2.323. [DOI] [PubMed] [Google Scholar]
  16. GOTTSCHALK C. W., MYLLE M. Micropuncture study of pressures in proximal tubules and peritubular capillaries of the rat kidney and their relation to ureteral and renal venous pressures. Am J Physiol. 1956 May;185(2):430–439. doi: 10.1152/ajplegacy.1956.185.2.430. [DOI] [PubMed] [Google Scholar]
  17. Gertz K. H., Mangos J. A., Braun G., Pagel H. D. On the glomerular tubular balance in the rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Sep 15;285(4):360–372. doi: 10.1007/BF00363236. [DOI] [PubMed] [Google Scholar]
  18. Gutknecht J. Salt transport in Valonia: inhibition of potassium uptake by small hydrostatic pressures. Science. 1968 Apr 5;160(3823):68–70. doi: 10.1126/science.160.3823.68. [DOI] [PubMed] [Google Scholar]
  19. Koch K. M., Aynedjian H. S., Bank N. Effect of acute hypertension on sodium reabsorption by the proximal tubule. J Clin Invest. 1968 Jul;47(7):1696–1709. doi: 10.1172/JCI105860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. LEVINSKY N. G., LALONE R. C. THE MECHANISM OF SODIUM DURESIS AFTER SALINE INFUSION IN THE DOG. J Clin Invest. 1963 Aug;42:1261–1276. doi: 10.1172/JCI104811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lewy J. E., Windhager E. E. Peritubular control of proximal tubular fluid reabsorption in the rat kidney. Am J Physiol. 1968 May;214(5):943–954. doi: 10.1152/ajplegacy.1968.214.5.943. [DOI] [PubMed] [Google Scholar]
  22. MILLS I. H., DE WARDENER H. E., HAYTER C. J., CLAPHAM W. F. Studies on the afferent mechanism of the sodium chloride diuresis which follows intravenous saline in the dog. Clin Sci. 1961 Oct;21:259–264. [PubMed] [Google Scholar]
  23. Martino J. A., Earley L. E. Demonstraton of a role of physical factors as determinants of the natriuretic response to volume expansion. J Clin Invest. 1967 Dec;46(12):1963–1978. doi: 10.1172/JCI105686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nutbourne D. M. The effect of small hydrostatic pressure gradients on the rate of active sodium transport across isolated living frog-skin membranes. J Physiol. 1968 Mar;195(1):1–18. doi: 10.1113/jphysiol.1968.sp008442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rector F. C., Jr, Brunner F. P., Seldin D. W. Mechanism of glomerulotubular balance. I. Effect of aortic constriction and elevated ureteropelvic pressure on glomerular filtration rate, fractional reabsorption, transit time, and tubular size in the proximal tubule of the rat. J Clin Invest. 1966 Apr;45(4):590–602. doi: 10.1172/JCI105373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rector F. C., Jr, Martinez-Maldonado M., Kurtzman N. A., Sellman J. C., Oerther F., Seldin D. W. Demonstration of a hormonal inhibitor of proximal tubular reabsorption during expansion of extracellular volume with isotonic saline. J Clin Invest. 1968 Apr;47(4):761–773. doi: 10.1172/JCI105771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rector F. C., Jr, Sellman J. C., Martinez-Maldonado M., Seldin D. W. The mechanism of suppression of proximal tubular reabsorption by saline infusions. J Clin Invest. 1967 Jan;46(1):47–56. doi: 10.1172/JCI105510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SELKURT E. E., HALL P. W., SPENCER M. P. Influence of graded arterial pressure decrement on renal clearance of creatinine, p-aminohippurate and sodium. Am J Physiol. 1949 Nov;159(2):369–378. doi: 10.1152/ajplegacy.1949.159.2.369. [DOI] [PubMed] [Google Scholar]
  29. STEIN R. M., BERCOVITCH D. D., LEVITT M. F. DUAL EFFECTS OF SALINE LOADING ON RENAL TUBULAR SODIUM REABSORPTION IN THE DOG. Am J Physiol. 1964 Oct;207:826–834. doi: 10.1152/ajplegacy.1964.207.4.826. [DOI] [PubMed] [Google Scholar]
  30. Smith H. W., Finkelstein N., Aliminosa L., Crawford B., Graber M. THE RENAL CLEARANCES OF SUBSTITUTED HIPPURIC ACID DERIVATIVES AND OTHER AROMATIC ACIDS IN DOG AND MAN. J Clin Invest. 1945 May;24(3):388–404. doi: 10.1172/JCI101618. [DOI] [PMC free article] [PubMed] [Google Scholar]

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