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. 2011 Oct 28;8:86. doi: 10.1186/1742-4690-8-86

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Copyright ©2011 Martin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HTLV-1 Tax (Tax1) and HTLV-2 Tax (Tax2) have different subcellular localization. pSG5M-Tax-1-His (left) and pSG5M-Tax-2B-His (right) were transiently transfected into coverglasses-seeded HeLa cells. Twenty four hours post-transfection, cells were fixed and stained with a rabbit anti-histidine antibody (sc-803, Santa Cruz Biotechnology) followed by a fluorescein-conjugated goat anti-rabbit antibody (green, Vector). Coverglasses were then mounted with DAPI-containing mounting medium (blue, Fluoromount-G, Southern Biotech) and cells were visualized using a Leica TCS SP5 confocal microscope. Images of cells that are representative of the entire population are shown.