Skip to main content
NCBI home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1969 Jul;48(7):1252–1257. doi: 10.1172/JCI106090

Internal redistribution of tissue protein synthesis in uremia

Leroy Shear 1
PMCID: PMC322347  PMID: 5794248

Abstract

Tissue composition and in vivo tissue protein synthesis were altered by acute uremia, induced in rats by bilateral nephrectomy. Net protein synthesis (anabolism minus catabolism) was increased in liver and heart and decreased in skeletal muscle, as judged from changes in total organ weight, ratios of protein: DNA and RNA: DNA, and leucine-14C incorporation into trichloroacetic acid (TCA)-insoluble, nucleic acid-free material. Concentration of free lysine, a major constituent of histones and ribonucleoproteins, also was increased in liver and decreased in skeletal muscle, a finding suggesting lysine shifted from muscle to liver in association with the changes in protein synthesis. Acute uremia also altered tissue levels of other amino acids. Hepatic concentrations and liver:blood concentration ratios tended to be increased for the essential, but not for the nonessential amino acids. Moreover, the phenylalanine:tyrosine concentration ratio, which reflects activity of the enzyme phenylalanine hydroxylase, was increased in blood, muscle, and liver. These findings indicate uremia selectively alters tissue composition and protein synthesis in different organs and may modify intermediary metabolism of some individual amino acids.

Full text

PDF
1252

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON J. B., WANNEMACHER R. W., Jr, BANKS W. L., Jr INFLUENCE OF DIETARY PROTEINS ON PROTEIN BIOSYNTHESIS IN VARIOUS TISSUES. Fed Proc. 1963 Jul-Aug;22:1126–1130. [PubMed] [Google Scholar]
  2. Anthony D. D., Zeszotek E., Goldthwait D. A. Initiation by the DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1966 Sep;56(3):1026–1033. doi: 10.1073/pnas.56.3.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Avioli L. V., Birge S., Lee S. W., Slatopolsky E. The metabolic fate of vitamin D3-3H in chronic renal failure. J Clin Invest. 1968 Oct;47(10):2239–2252. doi: 10.1172/JCI105909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bucher N. L. Experimental aspects of hepatic regeneration. N Engl J Med. 1967 Oct 5;277(14):738–concl. doi: 10.1056/NEJM196710052771405. [DOI] [PubMed] [Google Scholar]
  5. Clayton E. M., Seligson D., Seligson H. Inhibition of protein synthesis by N-methyl-2-pyridone-5-formamidoacetic acid and other compounds isolated from uremic patients. Yale J Biol Med. 1965 Dec;38(3):273–281. [PMC free article] [PubMed] [Google Scholar]
  6. DRABKIN D. L., MARSH J. B., BRAUN G. A. Amino acid mobilization in plasma protein biosynthesis in experimental nephrosis. Metabolism. 1962 Sep;11:967–977. [PubMed] [Google Scholar]
  7. Edozien J. C., Obasi M. E. Protein and amino acid metabolism in kwashiorkor. Clin Sci. 1965 Aug;29(1):1–24. [PubMed] [Google Scholar]
  8. FERRARI V., HARKNESS R. D. Free amino-acids in liver and blood after partial hepatectomy in normal and adrenalectomized rats. J Physiol. 1954 Jun 28;124(3):443–463. doi: 10.1113/jphysiol.1954.sp005120. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GOODFRIEND T. L., KAUFMAN S. Phenylalanine metabolism and folic acid antagonists. J Clin Invest. 1961 Sep;40:1743–1750. doi: 10.1172/JCI104397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gulyassy P. F., Peters J. H., Lin S. C., Ryan P. M. Hemodialysis and plasma amino acid composition in chronic renal failure. Am J Clin Nutr. 1968 Jun;21(6):565–573. doi: 10.1093/ajcn/21.6.565. [DOI] [PubMed] [Google Scholar]
  11. HUTCHISON W. C., MUNRO H. N. The determination of nucleic acids in biological materials. A review. Analyst. 1961 Dec;86:768–813. doi: 10.1039/an9618600768. [DOI] [PubMed] [Google Scholar]
  12. KIPNIS D. M., REISS E., HELMREICH E. Functional heterogeneity of the intracellular amino acid pool in mammalian cells. Biochim Biophys Acta. 1961 Aug 19;51:519–524. doi: 10.1016/0006-3002(61)90608-4. [DOI] [PubMed] [Google Scholar]
  13. McCormick G. J., Shear L., Barry K. G. Alteration of hepatic protein synthesis in acute uremia. Proc Soc Exp Biol Med. 1966 May;122(1):99–102. doi: 10.3181/00379727-122-31062. [DOI] [PubMed] [Google Scholar]
  14. YATVIN M. B., WANNEMACHER R. W., Jr ACTION OF ADRENAL CORTICOIDS ON PROTEIN METABOLISM IN THE THIOURACIL-TREATED RAT. Endocrinology. 1965 Mar;76:418–421. doi: 10.1210/endo-76-3-418. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES