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. 1969 Jul;48(7):1266–1272. doi: 10.1172/JCI106092

Characteristics of RNA degradation in the erythroid cell

Edward R Burka 1
PMCID: PMC322349  PMID: 5794250

Abstract

The characteristics of ribonucleic acid (RNA) degradation in intact reticulocytes have been investigated. The rate of degradation during in vitro maturation at 37°C is approximately 4% per hr. RNA degradation does not proceed at 0°C, and the rate of degradation is temperature dependent with an optimum at 50°C. The process is not dependent upon glycolysis. Although all types of RNA progressively decrease during in vivo maturation, ribosomes are degraded at a rate greater than that of soluble RNA.

RNA within the cell fluxes freely back and forth between the free and membrane-bound forms. The rate of loss of RNA in the membrane-bound compartment, both in the intact cell and in isolated membranes, is approximately threefold that found in the cell as a whole. During maturation the proportion of total cell RNA which is bound to the membrane decreases. The bulk of RNA destroyed is derived from the free compartment, but bound is also destroyed. The cell membrane participates in RNA metabolism and may be a site of RNA degradation in the erythroid cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADACHI K., NAGANO K., NAKAO T., NAKAO M. PURIFICATION AND CHARACTERIZATION OF RIBONUCLEASE FROM RABBIT RETICULOCYTES. Biochim Biophys Acta. 1964 Oct 23;92:59–70. doi: 10.1016/0926-6569(64)90269-x. [DOI] [PubMed] [Google Scholar]
  2. Aronson A. Adsorption of polysomes to bacterial membranes. J Mol Biol. 1966 Feb;15(2):505–514. doi: 10.1016/s0022-2836(66)80124-9. [DOI] [PubMed] [Google Scholar]
  3. Aronson A. Membrane-bound messenger RNA and polysomes in sporulating bacteria. J Mol Biol. 1965 Aug;13(1):92–104. doi: 10.1016/s0022-2836(65)80082-1. [DOI] [PubMed] [Google Scholar]
  4. BORSOOK H., LINGREL J. B., SCARO J. L., MILLETTE R. L. Synthesis of haemoglobin in relation to the maturation of erythroid cells. Nature. 1962 Oct 27;196:347–350. doi: 10.1038/196347a0. [DOI] [PubMed] [Google Scholar]
  5. BURKA E. R., MARKS P. A. PROTEIN SYNTHESIS IN ERYTHROID CELLS. II. POLYRIBOSOME FUNCTION IN INTACT RETICULOCYTES. J Mol Biol. 1964 Aug;9:439–451. doi: 10.1016/s0022-2836(64)80218-7. [DOI] [PubMed] [Google Scholar]
  6. Burka E. R., DeBellis R. Ribosome and polyribosome disappearance during in vivo erythroid maturation. Nature. 1967 Feb 18;213(5077):724–726. doi: 10.1038/213724a0. [DOI] [PubMed] [Google Scholar]
  7. Burka E. R. Determination of ribosenucleic acid in nonnucleated erythroid cells. J Lab Clin Med. 1966 Nov;68(5):833–837. [PubMed] [Google Scholar]
  8. Burka E. R., Schreml W., Kick C. J. Membrane-bound ribonucleic acid in mammalian erythroid cells. Biochemistry. 1967 Sep;6(9):2840–2847. doi: 10.1021/bi00861a026. [DOI] [PubMed] [Google Scholar]
  9. Burka E. R. The distribution of RNA and ribosomes in reticulocytes. Biochim Biophys Acta. 1968 Oct 29;166(3):672–680. doi: 10.1016/0005-2787(68)90374-2. [DOI] [PubMed] [Google Scholar]
  10. DEBELLIS R. H., GLUCK N., MARKS P. A. SYNTHESIS OF RIBONUCLEIC ACID IN RABBIT BLOOD CELLS IN VIVO. J Clin Invest. 1964 Jul;43:1329–1337. doi: 10.1172/JCI105008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Farkas W., Marks P. A. Partial purification and properties of a ribonuclease from rabbit reticulocytes. J Biol Chem. 1968 Dec 25;243(24):6464–6473. [PubMed] [Google Scholar]
  12. GRASSO J. A., WOODARD J. W., SWIFT H. Cytochemical studies of nucleic acids and proteins in erythrocytic development. Proc Natl Acad Sci U S A. 1963 Jul;50:134–140. doi: 10.1073/pnas.50.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LINDIGKEIT R. Die Nucleasewirksamkeit in den Reticulocyten von Kaninchen. Folia Haematol Int Mag Klin Morphol Blutforsch. 1956;74(3):251–257. [PubMed] [Google Scholar]
  14. MARKS P. A., RIFKIND R. A. DANON D: POLYRIBOSOMES AND PROTEIN SYNTHESIS DURING RETICULOCYTE MATURATION IN VITRO. Proc Natl Acad Sci U S A. 1963 Aug;50:336–342. doi: 10.1073/pnas.50.2.336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MATHIAS A. P., WILLIAMSON R., HUXLEY H. E., PAGE S. OCCURRENCE AND FUNCTION OF POLYSOMES IN RABBIT RETICULOCYTES. J Mol Biol. 1964 Jul;9:154–167. doi: 10.1016/s0022-2836(64)80097-8. [DOI] [PubMed] [Google Scholar]
  16. Marks P. A., Burka E. R., Schlessinger D. PROTEIN SYNTHESIS IN ERYTHROID CELLS, I. RETICULOCYTE RIBOSOMES ACTIVE IN STIMULATING AMINO ACID INCORPORATION. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2163–2171. doi: 10.1073/pnas.48.12.2163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morris A. J., Liang K. Interaction of globin and heme during hemoglobin biosynthesis. Arch Biochem Biophys. 1968 May;125(2):468–479. doi: 10.1016/0003-9861(68)90604-8. [DOI] [PubMed] [Google Scholar]
  18. NEU H. C., HEPPEL L. A. SOME OBSERVATIONS ON THE "LATENT" RIBONUCLEASE OF ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1964 Jun;51:1267–1274. doi: 10.1073/pnas.51.6.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RIFKIND R. A., DANON D., MARKS P. A. ALTERATIONS IN POLYRIBOSOMES DURING ERYTHROID CELL MATURATION. J Cell Biol. 1964 Sep;22:599–611. doi: 10.1083/jcb.22.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rowley P. T., Morris J. A. Protein synthesis in the maturing reticulocyte. J Biol Chem. 1967 Apr 10;242(7):1533–1540. [PubMed] [Google Scholar]
  21. STAVY L., FELDMAN M., ELSON D. ON RIBONUCLEASE ACTIVITY IN RETICULOCYTE RIBOSOMES. Biochim Biophys Acta. 1964 Dec 16;91:606–611. doi: 10.1016/0926-6550(64)90008-8. [DOI] [PubMed] [Google Scholar]
  22. Schreml W., Burka E. R. Properties of membrane-bound ribosomes in reticulocytes. J Biol Chem. 1968 Jul 10;243(13):3573–3580. [PubMed] [Google Scholar]
  23. Stonehill E. H., Huppert J. Ribonuclease activity associated with mammalian cell walls. Biochim Biophys Acta. 1968 Feb 26;155(2):353–359. doi: 10.1016/0005-2787(68)90179-2. [DOI] [PubMed] [Google Scholar]
  24. Wilner T., Izak G., Mager J. Observations on reticulocyte maturation. Isr J Med Sci. 1965 Jul;1(4):753–756. [PubMed] [Google Scholar]

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