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. 1969 Aug;48(8):1519–1531. doi: 10.1172/JCI106118

The relationship between peritubular capillary protein concentration and fluid reabsorption by the renal proximal tubule

Barry M Brenner 1, Kenneth H Falchuk 1, Robert I Keimowitz 1, Robert W Berliner 1
PMCID: PMC322379  PMID: 5796362

Abstract

The relationship between peritubular capillary protein concentration and rate of sodium reabsorption by the rat proximal tubule was examined using free-flow recollection micropuncture techniques. Tubule fluid-to-plasma inulin ratios were measured before, during, and at successive intervals after brief (15-25 sec) intra-aortic injections (at the level of the renal artery) of colloid-free, isoncotic, and hyperoncotic solutions. Arterial hematocrit and protein concentrations were measured simultaneously in these rats. In other rats, total protein concentration of peritubular capillary blood plasma was determined before, during, and after these same infusions with a newly described submicroliter fiber-optic colorimeter.

In the 15-25 sec interval necessary to infuse 2 ml of these test solutions, fractional and absolute sodium reabsorption varied directly with peritubular capillary colloid osmotic pressure, declining during infusion of colloid-free solutions, increasing during hyperoncotic infusions, and remaining unchanged during isoncotic infusions.

In the subsequent 20-min interval after intra-aortic injection of these test solutions, capillary protein concentration remained at (isoncotic infusions) or returned to (colloid-free and hyperoncotic fluids) control values. Whereas reabsorption after colloid-free solutions returned to base line levels in parallel with the return in capillary protein concentration, after colloid infusions (which resulted in continued expansion of extracellular fluid volume), a progressive decline in reabsorption was observed.

These results afford strong evidence that peritubular capillary colloid osmotic pressure is one important determinant of proximal sodium reabsorption. Nevertheless it is apparent that mechanisms other than or in addition to this must be invoked to explain the delayed inhibition of reabsorption that accompanies expansion of extracellular fluid volume by colloid solutions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRESLER E. H. The problem of the volume component of body fluid homeostasis. Am J Med Sci. 1956 Jul;232(1):93–104. doi: 10.1097/00000441-195607000-00014. [DOI] [PubMed] [Google Scholar]
  2. Brenner B. M., Bennett C. M., Berliner R. W. The relationship between glomerular filtration rate and sodium reabsorption by the proximal tubule of the rat nephron. J Clin Invest. 1968 Jun;47(6):1358–1374. doi: 10.1172/JCI105828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner B. M., Berliner R. W. Relationship between extracellular volume and fluid reabsorption by the rat nephron. Am J Physiol. 1969 Jul;217(1):6–12. doi: 10.1152/ajplegacy.1969.217.1.6. [DOI] [PubMed] [Google Scholar]
  4. Brunner F. P., Rector F. C., Jr, Seldin D. W. Mechanism of glomerulotubular balance. II. Regulation of proximal tubular reabsorption by tubular volume, as studied by stopped-flow microperfusion. J Clin Invest. 1966 Apr;45(4):603–611. doi: 10.1172/JCI105374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burg M. B., Orloff J. Control of fluid absorption in the renal proximal tubule. J Clin Invest. 1968 Sep;47(9):2016–2024. doi: 10.1172/JCI105888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DE WARDENER H. E., MILLS I. H., CLAPHAM W. F., HAYTER C. J. Studies on the efferent mechanism of the sodium diuresis which follows the administration of intravenous saline in the dog. Clin Sci. 1961 Oct;21:249–258. [PubMed] [Google Scholar]
  7. EARLEY L. E., FRIEDLER R. M. CHANGES IN RENAL BLOOD FLOW AND POSSIBLY THE INTRARENAL DISTRIBUTION OF BLOOD DURING THE NATRIURESIS ACCOMPANYING SALINE LOADING IN THE DOG. J Clin Invest. 1965 Jun;44:929–941. doi: 10.1172/JCI105210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Earley L. E., Friedler R. M. Studies on the mechanism of natriuresis accompanying increased renal blood flow and its role in the renal response to extracellular volume expansion. J Clin Invest. 1965 Nov;44(11):1857–1865. doi: 10.1172/JCI105293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Earley L. E., Friedler R. M. The effects of combined renal vasodilatation and pressor agents on renal hemodynamics and the tubular reabsorption of sodium. J Clin Invest. 1966 Apr;45(4):542–551. doi: 10.1172/JCI105368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Earley L. E., Martino J. A., Friedler R. M. Factors affecting sodium reabsorption by the proximal tubule as determined during blockade of distal sodium reabsorption. J Clin Invest. 1966 Nov;45(11):1668–1684. doi: 10.1172/JCI105474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FUHR J., KACZMARCZYK J., KRUTTGEN C. D. Eine einfache colorimetrische Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin Wochenschr. 1955 Aug 1;33(29-30):729–730. doi: 10.1007/BF01473295. [DOI] [PubMed] [Google Scholar]
  12. GIEBISCH G., KLOSE R. M., MALNIC G., SULLIVAN W. J., WINDHAGER E. E. SODIUM MOVEMENT ACROSS SINGLE PERFUSED PROXIMAL TUBULES OF RAT KIDNEYS. J Gen Physiol. 1964 Jul;47:1175–1194. doi: 10.1085/jgp.47.6.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GIEBISCH G., WINDHAGER E. E. RENAL TUBULAR TRANSFER OF SODIUM, CHLORIDE AND POTASSIUM. Am J Med. 1964 May;36:643–669. doi: 10.1016/0002-9343(64)90178-0. [DOI] [PubMed] [Google Scholar]
  14. Gertz K. H., Mangos J. A., Braun G., Pagel H. D. On the glomerular tubular balance in the rat kidney. Pflugers Arch Gesamte Physiol Menschen Tiere. 1965 Sep 15;285(4):360–372. doi: 10.1007/BF00363236. [DOI] [PubMed] [Google Scholar]
  15. Howards S. S., Davis B. B., Knox F. G., Wright F. S., Berliner R. W. Depression of fractional sodium reabsorption by the proximal tubule of the dog without sodium diuresis. J Clin Invest. 1968 Jul;47(7):1561–1572. doi: 10.1172/JCI105848. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Johnston C. I., Davis J. O. Evidence from cross circulation studies for a humoral mechanism in the natriuresis of saline loading. Proc Soc Exp Biol Med. 1966 Apr;121(4):1058–1063. doi: 10.3181/00379727-121-30965. [DOI] [PubMed] [Google Scholar]
  17. Johnston C. I., Davis J. O., Howards S. S., Wright F. S. Cross-circulation experiments on the mechanism of the natriuresis during saline loading in the dog. Circ Res. 1967 Jan;20(1):1–10. doi: 10.1161/01.res.20.1.1. [DOI] [PubMed] [Google Scholar]
  18. KASHGARIAN M., WARREN Y., MITCHELL R. L., EPSTEIN F. H. EFFECT OF PROTEIN IN TUBULAR FLUID UPON PROXIMAL TUBULAR ABSORPTION. Proc Soc Exp Biol Med. 1964 Dec;117:848–850. doi: 10.3181/00379727-117-29716. [DOI] [PubMed] [Google Scholar]
  19. KELMAN R. B. A theoretical note on exponential flow in the proximal part of the mammalian nephron. Bull Math Biophys. 1962 Sep;24:303–317. doi: 10.1007/BF02477961. [DOI] [PubMed] [Google Scholar]
  20. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  21. Levinsky N. G. Nonaldosterone influences on renal sodium transport. Ann N Y Acad Sci. 1966 Nov 22;139(2):295–303. doi: 10.1111/j.1749-6632.1966.tb41204.x. [DOI] [PubMed] [Google Scholar]
  22. Lewy J. E., Windhager E. E. Peritubular control of proximal tubular fluid reabsorption in the rat kidney. Am J Physiol. 1968 May;214(5):943–954. doi: 10.1152/ajplegacy.1968.214.5.943. [DOI] [PubMed] [Google Scholar]
  23. Martino J. A., Earley L. E. Demonstraton of a role of physical factors as determinants of the natriuretic response to volume expansion. J Clin Invest. 1967 Dec;46(12):1963–1978. doi: 10.1172/JCI105686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Martino J. A., Earley L. E. Relationship between intrarenal hydrostatic pressure and hemodynamically induced changes in sodium excretion. Circ Res. 1968 Sep;23(3):371–386. doi: 10.1161/01.res.23.3.371. [DOI] [PubMed] [Google Scholar]
  25. Rector F. C., Jr, Brunner F. P., Seldin D. W. Mechanism of glomerulotubular balance. I. Effect of aortic constriction and elevated ureteropelvic pressure on glomerular filtration rate, fractional reabsorption, transit time, and tubular size in the proximal tubule of the rat. J Clin Invest. 1966 Apr;45(4):590–602. doi: 10.1172/JCI105373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rector F. C., Jr, Martinez-Maldonado M., Kurtzman N. A., Sellman J. C., Oerther F., Seldin D. W. Demonstration of a hormonal inhibitor of proximal tubular reabsorption during expansion of extracellular volume with isotonic saline. J Clin Invest. 1968 Apr;47(4):761–773. doi: 10.1172/JCI105771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. VOGEL G., HEYM E., ANDERSSOHN K. Versuche zur Bedeutung kolloidosmotischer Druckdifferenzen für einen passiven Transportmechanismus in der Nierenkanälchen. Z Gesamte Exp Med. 1955;126(5):485–495. [PubMed] [Google Scholar]
  28. VOGEL G., HEYM E. Untersuchungen zur Bedeutung kolloidosomotischer Druckdifferenzen für den Mechanismus der isosmotischen Flüssigkeitsresorption in der Niere. Pflugers Arch. 1956;262(3):226–232. doi: 10.1007/BF00369703. [DOI] [PubMed] [Google Scholar]
  29. Vereerstraeten P., Toussaint C. Réduction de la natriurèse par la perfusion d'albumine dans la veine porte rénale du coq. Nephron. 1965;2(6):355–366. doi: 10.1159/000179417. [DOI] [PubMed] [Google Scholar]
  30. Vereerstraeten P., de Myttenaere M., Lambert P. P. Réduction de la natriurèse par la perfusion de protéines dans l'artère rénale du chien. Nephron. 1966;3(2):103–122. doi: 10.1159/000179451. [DOI] [PubMed] [Google Scholar]
  31. Vurek G. G., Bowman R. L. Fiber-optic colorimeter for submicroliter samples. Anal Biochem. 1969 May;29(2):238–247. doi: 10.1016/0003-2697(69)90307-8. [DOI] [PubMed] [Google Scholar]
  32. Wright F. S., Brenner B. M., Bennett C. M., Keimowitz R. I., Berliner R. W., Schrier R. W., Verroust P. J., De Wardener H. E., Holzgreve H. Failure to demonstrate a hormonal inhibitor of proximal sodium reabsorption. J Clin Invest. 1969 Jun;48(6):1107–1113. doi: 10.1172/JCI106067. [DOI] [PMC free article] [PubMed] [Google Scholar]

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