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. 1969 Oct;48(10):1855–1861. doi: 10.1172/JCI106151

The importance of aerobic metabolism in the renal concentrating process

Edward Weinstein 1, Andrzej Manitius 1, Franklin H Epstein 1
PMCID: PMC322421  PMID: 5822590

Abstract

The extent to which the concentrating function of the kidney depends on oxidative processes was investigated by infusing cyanide into one renal artery of dogs undergoing mild mannitol diuresis while receiving an infusion of vasopressin. This produced an abrupt fall in concentrating capacity (TcH2O) that was reversed when the cyanide infusion was stopped. The change could not be accounted for by the accompanying solute diuresis, since it was not reproduced by increasing the rate of mannitol infusion. The reduction in TcH2O induced by cyanide did not result from increased delivery of dilute urine to the collecting ducts, since free water clearance (CH2O), studied in other dogs during water diuresis, was unchanged or decreased by cyanide. Cyanide produced renal vasodilatation, as did intraarterial acetylcholine, but in contrast to the striking reduction in concentrating capacity evoked by cyanide, TcH2O was not significantly changed by acetylcholine.

The data indicate that concentrating ability is closely tied to oxidative metabolism in the kidney, and it is suggested that the region where this is critically important is the red medulla and the thick ascending limb of Henle's loop.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AUKLAND K., KROG J. Renal oxygen tension. Nature. 1960 Nov 19;188:671–671. doi: 10.1038/188671a0. [DOI] [PubMed] [Google Scholar]
  2. BERLYNE G. M., MACKEN A. On the mechanism of renal inability to produce a concentrated urine in chronic hydronephrosis. Clin Sci. 1962 Jun;22:315–324. [PubMed] [Google Scholar]
  3. BERNANKE D., EPSTEIN F. H. METABOLISM OF THE RENAL MEDULLA. Am J Physiol. 1965 Mar;208:541–545. doi: 10.1152/ajplegacy.1965.208.3.541. [DOI] [PubMed] [Google Scholar]
  4. Chertok R. J., Hulet W. H., Epstein B. Effects of cyanide, amytal, and DNP on renal sodium absorption. Am J Physiol. 1966 Dec;211(6):1379–1382. doi: 10.1152/ajplegacy.1966.211.6.1379. [DOI] [PubMed] [Google Scholar]
  5. Clapp J. R., Robinson R. R. Osmolality of distal tubular fluid in the dog. J Clin Invest. 1966 Dec;45(12):1847–1853. doi: 10.1172/JCI105488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Earley L. E., Friedler R. M. The effects of combined renal vasodilatation and pressor agents on renal hemodynamics and the tubular reabsorption of sodium. J Clin Invest. 1966 Apr;45(4):542–551. doi: 10.1172/JCI105368. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eknoyan G., Suki W. N., Rector F. C., Jr, Seldin D. W. Functional characteristics of the diluting segment of the dog nephron and the effect of extracellular volume expansion on its reabsorptive capacity. J Clin Invest. 1967 Jul;46(7):1178–1188. doi: 10.1172/JCI105611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Epstein F. H. Disorders of renal concentrating ability. Yale J Biol Med. 1966 Dec;39(3):186–195. [PMC free article] [PubMed] [Google Scholar]
  9. FUJIMOTO M., NASH F. D., KESSLER R. H. EFFECTS OF CYANIDE, QO, AND DINITROPHENOL ON RENAL SODIUM REABSORPTION AND OXYGEN CONSUMPTION. Am J Physiol. 1964 Jun;206:1327–1332. doi: 10.1152/ajplegacy.1964.206.6.1327. [DOI] [PubMed] [Google Scholar]
  10. HERMS W., MALVIN R. L. Effect of metabolic inhibitors on urine osmolality and electrolyte excretion. Am J Physiol. 1963 Jun;204:1065–1070. doi: 10.1152/ajplegacy.1963.204.6.1065. [DOI] [PubMed] [Google Scholar]
  11. JARAUSCH K. H., ULLRICH K. J. Untersuchungen zum Problem der Harnkonzentrierung und Harnverdünnung; Uber die Verteilung von Elektrolyten (Na, K, Ca, Mg, Cl, anorganischem Phosphat), Harnstoff, Aminosäuren und exogenem Kreatinin in Rinde und Mark der Hundeniere bei verschiedenen Diuresezuständen. Pflugers Arch. 1956;262(6):537–550. doi: 10.1007/BF00362116. [DOI] [PubMed] [Google Scholar]
  12. KEAN E. L., ADAMS P. H., DAVIES H. C., WINTERS R. W., DAVIES R. E. Oxygen consumption and respiratory pigments of mitochondria of the inner medulla of the dog kidney. Biochim Biophys Acta. 1962 Nov 5;64:503–507. doi: 10.1016/0006-3002(62)90308-6. [DOI] [PubMed] [Google Scholar]
  13. KEAN E. L., ADAMS P. H., WINTERS R. W., DAVIES R. E. Energy metabolism of the renal medulla. Biochim Biophys Acta. 1961 Dec 23;54:474–478. doi: 10.1016/0006-3002(61)90087-7. [DOI] [PubMed] [Google Scholar]
  14. LEE J. B., VANCE V. K., CAHILL G. F., Jr Metabolism of C14-labeled substrates by rabbit kidney cortex and medulla. Am J Physiol. 1962 Jul;203:27–36. doi: 10.1152/ajplegacy.1962.203.1.27. [DOI] [PubMed] [Google Scholar]
  15. PERILLIE P. E., EPSTEIN F. H. Sickling phenomenon produced by hypertonic solutions: a possible explanation for the hyposthenuria of sicklemia. J Clin Invest. 1963 Apr;42:570–580. doi: 10.1172/JCI104746. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. RHODIN J. Electron microscopy of the kidney. Am J Med. 1958 May;24(5):661–675. doi: 10.1016/0002-9343(58)90373-5. [DOI] [PubMed] [Google Scholar]
  17. STRICKLER J. C., KESSLER R. H. Effects of certain inhibitors on renal excretion of salt and water. Am J Physiol. 1963 Jul;205:117–122. doi: 10.1152/ajplegacy.1963.205.1.117. [DOI] [PubMed] [Google Scholar]
  18. ULLRICH K. J. Aktiver Natriumtransport und Sauerstoffverbrauch in der äusseren Markzone der Niere. Pflugers Arch. 1958;267(3):207–217. doi: 10.1007/BF00362425. [DOI] [PubMed] [Google Scholar]
  19. WACHSTEIN M. Histochemical staining reactions of the normally functioning and abnormal kidney. J Histochem Cytochem. 1955 Jul;3(4):246–270. doi: 10.1177/3.4.246. [DOI] [PubMed] [Google Scholar]
  20. WALSER M., DAVIDSON D. G., ORLOFF J. The renal clearance of alkali-stable inulin. J Clin Invest. 1955 Oct;34(10):1520–1523. doi: 10.1172/JCI103204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WINDHAGER E. E. ELECTROPHYSIOLOGICAL STUDY OF RENAL PAPILLA OF GOLDEN HAMSTERS. Am J Physiol. 1964 Apr;206:694–700. doi: 10.1152/ajplegacy.1964.206.4.694. [DOI] [PubMed] [Google Scholar]
  22. Washington J. A., 2nd, Holland J. M. Urine oxygen tension: effects of osmotic and saline diuresis and of ethacrynic acid. Am J Physiol. 1966 Feb;210(2):243–250. doi: 10.1152/ajplegacy.1966.210.2.243. [DOI] [PubMed] [Google Scholar]

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