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. 1970 Jan;49(1):63–77. doi: 10.1172/JCI106224

Effect of lidocaine on the electrophysiological properties of ventricular muscle and Purkinje fibers

J Thomas Bigger Jr 1,2, William J Mandel 1,2
PMCID: PMC322445  PMID: 5409809

Abstract

Preparations of right ventricular papillary muscle and false tendon (Purkinje fiber) were obtained from dog hearts, placed in a bath perfused with Tyrode solution, and observed both under control conditions and during exposure to lidocaine in concentrations from 1 × 10-7 to 5 × 10-4 mole/liter. Transmembrane voltages were recorded from both ventricular muscle (VM) and Purkinje fibers (PF) of spontaneously beating and electrically driven preparations. Low concentrations (1 × 10-6 and 1 × 10-5 mole/liter) attenuated or abolished phase 4 (diastolic) depolarization and spontaneous firing in PF without decreasing their diastolic excitability. Concentrations of 1 × 10-5 mole/liter produced maximal shortening of both action potential duration (APD) and effective refractory period (ERP) and made the ERP long relative to APD; the latter alteration was more prominent in VM. At concentrations ≤ 1 × 10-5 mole/liter, lidocaine either caused a slight increase or no change in peak maximum rate of phase 0 depolarization (Vmax) and membrane responsiveness, the relationship between transmembrane activation voltage (MAV) and Vmax of the resultant action potential; these concentrations had no significant effect on resting potential (RP) in VM, maximal diastolic transmembrane voltage (DTMVmax) in PF, or action potential amplitude in either fiber type.

High (toxic) concentrations (≥ 1 × 10-4 mole/liter) did not cause further shortening of APD or ERP in either VM or PF but did produce a decrease in peak Vmax of phase 0 and membrane responsiveness. In most cases, these concentrations also caused a decrease in RP or DTMVmax and action potential amplitude, with progression to bizarre action potential depolarization and inexcitability. These properties of lidocaine are strikingly different from those of quinidine or procaine amide. The mechanisms responsible for lidocaine's in vivo antiarrhythmic action are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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