Skip to main content
PMC home page
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1970 Apr;49(4):655–665. doi: 10.1172/JCI106277

The measurement of the exchangeable pools of cholesterol in the baboon

Jean D Wilson 1
PMCID: PMC322520  PMID: 4986214

Abstract

Five male baboons were fed cholesterol-1α-3H until an isotopic steady state was approached in which the ratio of serum to dietary cholesterol specific activity was constant. The animals were then given single intravenous injections of cholesterol-4-14C, and the disappearance curves of the 14C from the circulation were followed for 70-85 days. The total exchangeable cholesterol pools under these conditions were determined by carcass analysis at the end of the experiment and were shown to agree closely with the sums of the two exchangeable cholesterol pools as predicted from analysis of the die-away curve in terms of a two exchangeable pool model, assuming that entry into and exit from the system occur predominantly through the more rapidly exchanging of the two pools. These results have been interpreted as support for the validity of a model consisting of two exchangeable pools and one nonexchangeable or very slowly exchangeable pool as an approximation of over-all cholesterol metabolism in this species. In addition, an anatomical and chemical dissection of these pools was performed. While the three pool model is not applicable to every tissue, it has been possible to show that its general applicability in the intact animal stems from the fact that the major portions of the three cholesterol pools of the body lie within a limited number of tissues.

Full text

PDF
655

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHOBANIAN A. V., HOLLANDER W. Body cholesterol metabolism in man. I. The equilibration of serum and tissue cholesterol. J Clin Invest. 1962 Sep;41:1732–1737. doi: 10.1172/JCI104631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CONNOR W. E., HODGES R. E., BLEILER R. E. The serum lipids in men receiving high cholesterol and cholesterol-free diets. J Clin Invest. 1961 May;40:894–901. doi: 10.1172/JCI104324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dietschy J. M., Wilson J. D. Cholesterol synthesis in the squirrel monkey: relative rates of synthesis in various tissues and mechanisms of control. J Clin Invest. 1968 Jan;47(1):166–174. doi: 10.1172/JCI105706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FIELD H., Jr, SWELL L., SCHOOLS P. E., Jr, TREADWELL C. R. Dynamic aspects of cholesterol metabolism in different areas of the aorta and other tissues in man and their relationship to atherosclerosis. Circulation. 1960 Oct;22:547–558. doi: 10.1161/01.cir.22.4.547. [DOI] [PubMed] [Google Scholar]
  5. GURPIDE E., MANN J., LIEBERMAN S. ANALYSIS OF OPEN SYSTEMS OF MULTIPLE POOLS BY ADMINISTRATION OF TRACERS AT A CONSTANT RATE OR AS A SINGLE DOSE AS ILLUSTRATED BY PROBLEMS INVOLVING STEROID HORMONES. J Clin Endocrinol Metab. 1963 Nov;23:1155–1176. doi: 10.1210/jcem-23-11-1155. [DOI] [PubMed] [Google Scholar]
  6. GURPIDE E., MANN J., SANDBERG E. DETERMINATION OF KINETIC PARAMETERS INA TWO-POOL SYSTEM BY ADMINISTRATION OF ONE OR MORE TRACERS. Biochemistry. 1964 Sep;3:1250–1255. doi: 10.1021/bi00897a012. [DOI] [PubMed] [Google Scholar]
  7. Goodman D. S., Noble R. P. Turnover of plasma cholesterol in man. J Clin Invest. 1968 Feb;47(2):231–241. doi: 10.1172/JCI105719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grundy S. M., Ahrens E. H., Jr Measurements of cholesterol turnover, synthesis, and absorption in man, carried out by isotope kinetic and sterol balance methods. J Lipid Res. 1969 Jan;10(1):91–107. [PubMed] [Google Scholar]
  9. LINDSEY C. A., Jr, WILSON J. D. EVIDENCE FOR A CONTRIBUTION BY THE INTESTINAL WALL TO THE SERUM CHOLESTEROL OF THE RAT. J Lipid Res. 1965 Apr;6:173–181. [PubMed] [Google Scholar]
  10. MEIER J. R., SIPERSTEIN M. D., CHAIKOFF I. L. C14-Cholesterol. IV. Oxidation of carbons 4 and 26 to carbon dioxide by surviving tissues. J Biol Chem. 1952 Sep;198(1):105–109. [PubMed] [Google Scholar]
  11. Nestel P. J., Whyte H. M., Goodman D. S. Distribution and turnover of cholesterol in humans. J Clin Invest. 1969 Jun;48(6):982–991. doi: 10.1172/JCI106079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SPERRY W. M. QUANTITATIVE ISOLATION OF STEROLS. J Lipid Res. 1963 Apr;4:221–225. [PubMed] [Google Scholar]
  13. SPERRY W. M., WEBB M. A revision of the Schoenheimer-Sperry method for cholesterol determination. J Biol Chem. 1950 Nov;187(1):97–106. [PubMed] [Google Scholar]
  14. Samuel P., Holtzman C. H., Meilman E., Perl W. Effect of neomycin on exchangeable pools of cholesterol in the steady state. J Clin Invest. 1968 Aug;47(8):1806–1818. doi: 10.1172/JCI105870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wilson J. D. Biosynthetic origin of serum cholesterol in the squirrel monkey: evidence for a contribution by the intestinal wall. J Clin Invest. 1968 Jan;47(1):175–187. doi: 10.1172/JCI105707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wilson J. D., Lindsey C. A., Jr Studies on the influence of dietary cholesterol on cholesterol metabolism in the isotopic steady state in man. J Clin Invest. 1965 Nov;44(11):1805–1814. doi: 10.1172/JCI105288. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES