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. 2011 Oct 1;6(10):1198–1206. doi: 10.4161/epi.6.10.16600

Figure 4.

Figure 4

Leptin expression in skin fibroblasts and HeLa. (A, C) Representative images of human primary skin fibroblasts (A) and HeLa cells (C) cultured in normal conditions (Control) and upon decitabine (DAC) treatment, which caused an inhibition of cell growth. Fibroblasts and Hela cells were respectively treated for 4 and 3 days with 100 µM DAC. Calibration bars, 50 µm. (B,D) Leptin expression in DAC versus untreated cells, quantified by Real-Time PCR and normalized against that of TBP. SE, error bars. t-test, *p < 0.05. For each condition at least five wells were considered.