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. 1970 Jul;49(7):1394–1404. doi: 10.1172/JCI106357

Synthesis and release of proinsulin and insulin by isolated rat islets of Langerhans

Tomio Tanese 1,2, Norman R Lazarus 1,2, Sevim Devrim 1,2, Lillian Recant 1,2
PMCID: PMC322613  PMID: 4914679

Abstract

Isolated rat islets of Langerhans were incubated for 60, 120, and 180 min and the incorporation of leucine-3H into proinsulin and insulin moieties was followed. Synthesis and release of these hormones could be followed by separate extractions of islets and incubation media.

Release of newly synthesized proinsulin and insulin occurred under the following conditions: (a) incubation for greater than 60 min; (b) glucose concentrations above 5.3 mmoles/liter; (c) incubation with 5 mM dibutyryl cyclic AMP or theophylline in 5.3 mM glucose (potentiated by 16 mM glucose); and (d) incubation with 5 mM tolbutamide and 16 mM glucose.

Synthesis of proinsulin and insulin was enhanced by time of incubation, high glucose concentrations, by dibutyryl cyclic AMP or theophylline, and by tolbutamide only at 16 mM glucose. Synthesis was totally inhibited by tolbutamide at 5.3 mM glucose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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