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Composition of S100A4–myosin-IIA complexes. (A) Coomassie-stained SDS–PAGE of 12.5 μM wt-S100A4 dimer and 25μM MIIA monomer in the presence of DST, a 6.4 Å cross-linker. Asterisks denote complexes observed in the presence of calcium. (B) Western blots of cross-linked samples. Duplicate protein samples were separated by SDS–PAGE and transferred to a PVDF membrane, which was cut in half and probed with antibodies to either S100A4 or myosin-IIA.
