Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Jan;83(1):165–169. doi: 10.1073/pnas.83.1.165

Continuous production of erythropoietin by an established human renal carcinoma cell line: development of the cell line.

J B Sherwood, D Shouval
PMCID: PMC322812  PMID: 3455754

Abstract

Establishment of a stable, transformed human renal carcinoma cell line that produces erythropoietin in vitro and has maintained this function continuously since 1981 and for greater than 150 passages in monolayer culture was accomplished by transplantation of human renal clear cell carcinoma tissue from a patient with erythrocytosis into an immunosuppressed athymic mouse. In addition to its immunocrossreactivity with native human urinary erythropoietin, the tumor erythropoietin demonstrates biological activity in the in vitro mouse erythroid colony-forming unit assay and in tumor-bearing nude mice. The cloned renal carcinoma cell line has an abnormal human karyotype and has ultrastructural features characteristic of human renal clear cell carcinoma. This cell line provides a reproducible model system for the production of an erythropoietin-like material and for the study of its synthesis and secretion.

Full text

PDF
165

Images in this article

166Image
on p.166
167Image
on p.167

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ascensao J. L., Gaylis F., Bronson D., Fraley E. E., Zanjani E. D. Erythropoietin production by a human testicular germ cell line. Blood. 1983 Nov;62(5):1132–1134. [PubMed] [Google Scholar]
  2. Burlington H., Cronkite E. P., Reincke U., Zanjani E. D. Erythropoietin production in cultures of goat renal glomeruli. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3547–3550. doi: 10.1073/pnas.69.12.3547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Choppin J., Lacombe C., Casadevall N., Muller O., Tambourin P., Varet B. Characterization of erythropoietin produced by IW32 murine erythroleukemia cells. Blood. 1984 Aug;64(2):341–347. [PubMed] [Google Scholar]
  4. Hagiwara M., Chen I. L., McGonigle R., Beckman B., Kasten F. H., Fisher J. W. Erythropoietin production in a primary culture of human renal carcinoma cells maintained in nude mice. Blood. 1984 Apr;63(4):828–835. [PubMed] [Google Scholar]
  5. Iscove N. N., Sieber F., Winterhalter K. H. Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. J Cell Physiol. 1974 Apr;83(2):309–320. doi: 10.1002/jcp.1040830218. [DOI] [PubMed] [Google Scholar]
  6. Klinger H. P. Rapid processing of primary embryonic tissues for chromosome banding pattern analysis. Cytogenetics. 1972;11(5):424–435. doi: 10.1159/000130208. [DOI] [PubMed] [Google Scholar]
  7. Kurtz A., Jelkmann W., Sinowatz F., Bauer C. Renal mesangial cell cultures as a model for study of erythropoietin production. Proc Natl Acad Sci U S A. 1983 Jul;80(13):4008–4011. doi: 10.1073/pnas.80.13.4008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lappin T. R., Rich I., Goldwasser E. The effect of erythropoietin and other factors on DNA synthesis by mouse spleen cells. Exp Hematol. 1983 Aug;11(7):661–666. [PubMed] [Google Scholar]
  10. MACPHERSON I., MONTAGNIER L. AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS. Virology. 1964 Jun;23:291–294. doi: 10.1016/0042-6822(64)90301-0. [DOI] [PubMed] [Google Scholar]
  11. McDonald T. P., Martin D. H., Simmons M. L., Lange R. D. Preliminary results of erythropoietin production by bovine kidney cells in culture. Life Sci. 1969 Sep 15;8(18):949–954. doi: 10.1016/0024-3205(69)90199-4. [DOI] [PubMed] [Google Scholar]
  12. Miyake T., Kung C. K., Goldwasser E. Purification of human erythropoietin. J Biol Chem. 1977 Aug 10;252(15):5558–5564. [PubMed] [Google Scholar]
  13. Ogle J. W., Lange R. D., Dunn C. D. Production of erythropoietin in vitro: a review. In Vitro. 1978 Nov;14(11):945–950. doi: 10.1007/BF02616125. [DOI] [PubMed] [Google Scholar]
  14. Sherwood J. B., Goldwasser E. A radioimmunoassay for erythropoietin. Blood. 1979 Oct;54(4):885–893. [PubMed] [Google Scholar]
  15. Sherwood J. B., Goldwasser E. Erythropoietin production by human renal carcinoma cells in culture. Endocrinology. 1976 Aug;99(2):504–510. doi: 10.1210/endo-99-2-504. [DOI] [PubMed] [Google Scholar]
  16. Sherwood J. B., Goldwasser E. Extraction of erythropoietin from normal kidneys. Endocrinology. 1978 Sep;103(3):866–870. doi: 10.1210/endo-103-3-866. [DOI] [PubMed] [Google Scholar]
  17. Sherwood J. B., Robinson S. H., Bassan L. R., Rosen S., Gordon A. S. Production of erythrogenin by organ cultures of rat kidney. Blood. 1972 Aug;40(2):189–197. [PubMed] [Google Scholar]
  18. Shouval D., Rager-Zisman B., Quan P., Shafritz D. A., Bloom B. R., Reid L. M. Role in nude mice of interferon and natural killer cells in inhibiting the tumorigenicity of human hepatocellular carcinoma cells infected with hepatitis B virus. J Clin Invest. 1983 Aug;72(2):707–717. doi: 10.1172/JCI111020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shouval D., Reid L. M., Chakraborty P. R., Ruiz-Opazo N., Morecki R., Gerber M. A., Thung S. N., Shafritz D. A. Tumorigenicity in nude mice of a human hepatoma cell line containing hepatitis B virus DNA. Cancer Res. 1981 Apr;41(4):1342–1350. [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Worton R. G., Duff C. Karyotyping. Methods Enzymol. 1979;58:322–344. doi: 10.1016/s0076-6879(79)58148-8. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES