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. 1986 Jan;83(2):265–269. doi: 10.1073/pnas.83.2.265

Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins.

S M Mumby, R A Kahn, D R Manning, A G Gilman
PMCID: PMC322838  PMID: 3079909

Abstract

Antisera were raised against purified subunits of regulatory GTP-binding proteins (G proteins) and against synthetic peptides that correspond to defined regions of G proteins. Peptide antisera were generated that recognized all alpha or all beta subunits from Gs, Gi, Go, and transducin; others recognized only Gs alpha or Go alpha. Such cross-reaction or complete specificity for a given alpha subunit was not obtained when purified subunits were injected. Peptide antisera were used to identify G protein subunits in selected tissue membrane preparations by immunoblots.

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