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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Jan;83(2):352–356. doi: 10.1073/pnas.83.2.352

Light-scattering study of depolymerization kinetics of sickle hemoglobin polymers inside single erythrocytes.

J Peetermans, I Nishio, S T Ohnishi, T Tanaka
PMCID: PMC322856  PMID: 3455772

Abstract

The time course of intracellular depolymerization of hemoglobin S aggregates is directly observed by using microscope laser light-scattering spectroscopy in single sickle erythrocytes upon slow reoxygenation. From the correlation functions of the light intensity scattered from a single cell, we determine the average diffusion coefficient as well as the fraction of hemoglobin aggregates that have intracellular mobility. The oxygen saturation of the hemoglobin of the same cell is measured by single-cell absorption spectrophotometry. Combining the results obtained with these techniques and information on the cellular morphology, we propose a model for the depolymerization process of hemoglobin inside single erythrocytes as they transform from the fully sickled to the normal biconcave shape upon reoxygenation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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