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. 1986 Jan;83(2):517–521. doi: 10.1073/pnas.83.2.517

Different types of Ca2+ channels in mammalian skeletal muscle cells in culture.

C Cognard, M Lazdunski, G Romey
PMCID: PMC322891  PMID: 2417248

Abstract

This paper describes the existence of two pharmacologically distinct types of Ca2+ channels in rat skeletal muscle cells (myoballs) in culture. The first class of Ca2+ channels is insensitive to the dihydropyridine (DHP) (+)-PN 200-110; the second class of Ca2+ channels is blocked by low concentrations of (+)-PN 200-110. The two pharmacologically different Ca2+ channels are also different in their voltage and time dependence. The threshold for activation of the DHP-insensitive Ca2+ channel is near -65 mV, whereas the threshold for activation of the DHP-sensitive Ca2+ channel is near -30 mV. Current flowing through the DHP-insensitive Ca2+ channel is transient with relatively fast kinetics. Half-maximal inactivation for the DHP-insensitive Ca2+ channel is observed at a holding potential Vh0.5 = -78 mV and the channel is completely inactivated at -60 mV. Two different behaviors have been found for DHP-sensitive channels with two different kinetics of inactivation (one being about 16 times faster than the other at -2 mV) and two different voltage dependencies. These two different behaviors are often observed in the same myoball and may correspond to two different subtypes of DHP-sensitive Ca2+ channels or to two different modes of expression of one single Ca2+ channel protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almers W., McCleskey E. W. Non-selective conductance in calcium channels of frog muscle: calcium selectivity in a single-file pore. J Physiol. 1984 Aug;353:585–608. doi: 10.1113/jphysiol.1984.sp015352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Almers W., Palade P. T. Slow calcium and potassium currents across frog muscle membrane: measurements with a vaseline-gap technique. J Physiol. 1981 Mar;312:159–176. doi: 10.1113/jphysiol.1981.sp013622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boll W., Lux H. D. Action of organic antagonists on neuronal calcium currents. Neurosci Lett. 1985 May 23;56(3):335–339. doi: 10.1016/0304-3940(85)90265-4. [DOI] [PubMed] [Google Scholar]
  4. Borsotto M., Barhanin J., Fosset M., Lazdunski M. The 1,4-dihydropyridine receptor associated with the skeletal muscle voltage-dependent Ca2+ channel. Purification and subunit composition. J Biol Chem. 1985 Nov 15;260(26):14255–14263. [PubMed] [Google Scholar]
  5. Borsotto M., Barhanin J., Norman R. I., Lazdunski M. Purification of the dihydropyridine receptor of the voltage-dependent Ca2+ channel from skeletal muscle transverse tubules using (+) [3H]PN 200-110. Biochem Biophys Res Commun. 1984 Aug 16;122(3):1357–1366. doi: 10.1016/0006-291x(84)91241-5. [DOI] [PubMed] [Google Scholar]
  6. Bossu J. L., Feltz A., Thomann J. M. Depolarization elicits two distinct calcium currents in vertebrate sensory neurones. Pflugers Arch. 1985 Apr;403(4):360–368. doi: 10.1007/BF00589247. [DOI] [PubMed] [Google Scholar]
  7. Carbone E., Lux H. D. A low voltage-activated calcium conductance in embryonic chick sensory neurons. Biophys J. 1984 Sep;46(3):413–418. doi: 10.1016/S0006-3495(84)84037-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carbone E., Lux H. D. A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones. Nature. 1984 Aug 9;310(5977):501–502. doi: 10.1038/310501a0. [DOI] [PubMed] [Google Scholar]
  9. DeHaan R. L. Differentiation of excitable membranes. Curr Top Dev Biol. 1980;16:117–164. doi: 10.1016/s0070-2153(08)60155-6. [DOI] [PubMed] [Google Scholar]
  10. Fedulova S. A., Kostyuk P. G., Veselovsky N. S. Two types of calcium channels in the somatic membrane of new-born rat dorsal root ganglion neurones. J Physiol. 1985 Feb;359:431–446. doi: 10.1113/jphysiol.1985.sp015594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Fishman M. C., Spector I. Potassium current suppression by quinidine reveals additional calcium currents in neuroblastoma cells. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5245–5249. doi: 10.1073/pnas.78.8.5245. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fosset M., Jaimovich E., Delpont E., Lazdunski M. [3H]nitrendipine receptors in skeletal muscle. J Biol Chem. 1983 May 25;258(10):6086–6092. [PubMed] [Google Scholar]
  13. Frelin C., Vigne P., Lazdunski M. Na+ channels with high and low affinity tetrodotoxin binding sites in the mammalian skeletal muscle cell. Difference in functional properties and sequential appearance during rat skeletal myogenesis. J Biol Chem. 1983 Jun 25;258(12):7256–7259. [PubMed] [Google Scholar]
  14. Frelin C., Vigne P., Schweitz H., Lazdunski M. The interaction of sea anemone and scorpion neurotoxins with tetrodotoxin-resistant Na+ channels in rat myoblasts. A comparison with Na+ channels in other excitable and non-excitable cells. Mol Pharmacol. 1984 Jul;26(1):70–74. [PubMed] [Google Scholar]
  15. Grampp W., Harris J. B., Thesleff S. Inhibition of denervation changes in skeletal muscle by blockers of protein synthesis. J Physiol. 1972 Mar;221(3):743–754. doi: 10.1113/jphysiol.1972.sp009780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hamill O. P., Marty A., Neher E., Sakmann B., Sigworth F. J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. doi: 10.1007/BF00656997. [DOI] [PubMed] [Google Scholar]
  17. Hess P., Lansman J. B., Tsien R. W. Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists. Nature. 1984 Oct 11;311(5986):538–544. doi: 10.1038/311538a0. [DOI] [PubMed] [Google Scholar]
  18. Ildefonse M., Jacquemond V., Rougier O., Renaud J. F., Fosset M., Lazdunski M. Excitation contraction coupling in skeletal muscle: evidence for a role of slow Ca2+ channels using Ca2+ channel activators and inhibitors in the dihydropyridine series. Biochem Biophys Res Commun. 1985 Jun 28;129(3):904–909. doi: 10.1016/0006-291x(85)91977-1. [DOI] [PubMed] [Google Scholar]
  19. Llinás R., Yarom Y. Properties and distribution of ionic conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro. J Physiol. 1981 Jun;315:569–584. doi: 10.1113/jphysiol.1981.sp013764. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Miller R. J., Freedman S. B. Are dihydropyridine binding sites voltage sensitive calcium channels? Life Sci. 1984 Mar 26;34(13):1205–1221. doi: 10.1016/0024-3205(84)90543-5. [DOI] [PubMed] [Google Scholar]
  21. Nilius B., Hess P., Lansman J. B., Tsien R. W. A novel type of cardiac calcium channel in ventricular cells. Nature. 1985 Aug 1;316(6027):443–446. doi: 10.1038/316443a0. [DOI] [PubMed] [Google Scholar]
  22. Nowycky M. C., Fox A. P., Tsien R. W. Long-opening mode of gating of neuronal calcium channels and its promotion by the dihydropyridine calcium agonist Bay K 8644. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2178–2182. doi: 10.1073/pnas.82.7.2178. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nowycky M. C., Fox A. P., Tsien R. W. Three types of neuronal calcium channel with different calcium agonist sensitivity. Nature. 1985 Aug 1;316(6027):440–443. doi: 10.1038/316440a0. [DOI] [PubMed] [Google Scholar]
  24. Pappone P. A. Voltage-clamp experiments in normal and denervated mammalian skeletal muscle fibres. J Physiol. 1980 Sep;306:377–410. doi: 10.1113/jphysiol.1980.sp013403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Potreau D., Raymond G. Slow inward barium current and contraction on frog single muscle fibres. J Physiol. 1980 Jun;303:91–109. doi: 10.1113/jphysiol.1980.sp013273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Romey G., Lazdunski M. Lipid-soluble toxins thought to be specific for Na+ channels block Ca2+ channels in neuronal cells. Nature. 1982 May 6;297(5861):79–78. doi: 10.1038/297079a0. [DOI] [PubMed] [Google Scholar]
  27. Sanchez J. A., Stefani E. Inward calcium current in twitch muscle fibres of the frog. J Physiol. 1978 Oct;283:197–209. doi: 10.1113/jphysiol.1978.sp012496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Schmid-Antomarchi H., Renaud J. F., Romey G., Hugues M., Schmid A., Lazdunski M. The all-or-none role of innervation in expression of apamin receptor and of apamin-sensitive Ca2+-activated K+ channel in mammalian skeletal muscle. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2188–2191. doi: 10.1073/pnas.82.7.2188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schwartz L. M., McCleskey E. W., Almers W. Dihydropyridine receptors in muscle are voltage-dependent but most are not functional calcium channels. 1985 Apr 25-May 1Nature. 314(6013):747–751. doi: 10.1038/314747a0. [DOI] [PubMed] [Google Scholar]
  30. Thesleff S. Trophic functions of the neuron. II. Denervation and regulation of muscle. Physiological effects of denervation of muscle. Ann N Y Acad Sci. 1974 Mar 22;228(0):89–104. doi: 10.1111/j.1749-6632.1974.tb20504.x. [DOI] [PubMed] [Google Scholar]
  31. Thesleff S., Ward M. R. Studies on the mechanism of fibrillation potentials in denervated muscle. J Physiol. 1975 Jan;244(2):313–323. doi: 10.1113/jphysiol.1975.sp010800. [DOI] [PMC free article] [PubMed] [Google Scholar]

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