Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Feb;83(3):591–593. doi: 10.1073/pnas.83.3.591

Distinctive role of histidine-16 of the B beta chain of fibrinogen in the end-to-end association of fibrin.

A Shimizu, Y Saito, Y Inada
PMCID: PMC322909  PMID: 3456154

Abstract

Photooxidation of fibrinogen reduced the batroxobin-induced fibrin polymerization. The fibrin fragment des-AB N-DSK, which contains the binding sites termed A and B, lost the ability to bind to the site termed a in fibrinogen-Sepharose upon the oxidation of histidine-16 in the B beta chain of fibrinogen [Shimizu, A., Saito, Y., Matsushima, A. & Inada, Y. (1983) J. Biol. Chem. 258, 7915-7917]. Some of the fragments, which became unable to bind to fibrinogen-Sepharose due to the destruction of site A, however, retained the ability to bind to D-dimer-Sepharose, which contains both sites a and b. This shows that histidine-16 of the B beta chain of fibrinogen is essential for site A but may not be essential for site B. It is of interest that histidine-16 of the B beta chain, which is only one residue away from the thrombin-susceptible bond, makes a part of the site A for the end-to-end association created by the release of fibrinopeptide A.

Full text

PDF
591

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blombäck B., Hessel B., Hogg D., Therkildsen L. A two-step fibrinogen--fibrin transition in blood coagulation. Nature. 1978 Oct 12;275(5680):501–505. doi: 10.1038/275501a0. [DOI] [PubMed] [Google Scholar]
  2. Blombäck M., Blombäck B., Mammen E. F., Prasad A. S. Fibrinogen Detroit--a molecular defect in the N-terminal disulphide knot of human fibrinogen? Nature. 1968 Apr 13;218(5137):134–137. doi: 10.1038/218134a0. [DOI] [PubMed] [Google Scholar]
  3. Hoeprich P. D., Jr, Doolittle R. F. Dimeric half-molecules of human fibrinogen are joined through disulfide bonds in an antiparallel orientation. Biochemistry. 1983 Apr 26;22(9):2049–2055. doi: 10.1021/bi00278a003. [DOI] [PubMed] [Google Scholar]
  4. Horwitz B. H., Váradi A., Scheraga H. A. Localization of a fibrin gamma-chain polymerization site within segment Thr-374 to Glu-396 of human fibrinogen. Proc Natl Acad Sci U S A. 1984 Oct;81(19):5980–5984. doi: 10.1073/pnas.81.19.5980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kudryk B. J., Collen D., Woods K. R., Blombäck B. Evidence for localization of polymerization sites in fibrinogen. J Biol Chem. 1974 May 25;249(10):3322–3325. [PubMed] [Google Scholar]
  6. Laudano A. P., Doolittle R. F. Synthetic peptide derivatives that bind to fibrinogen and prevent the polymerization of fibrin monomers. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3085–3089. doi: 10.1073/pnas.75.7.3085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Olexa S. A., Budzynski A. Z. Binding phenomena of isolated unique plasmic degradation products of human cross-linked fibrin. J Biol Chem. 1979 Jun 10;254(11):4925–4932. [PubMed] [Google Scholar]
  8. Olexa S. A., Budzynski A. Z. Evidence for four different polymerization sites involved in human fibrin formation. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1374–1378. doi: 10.1073/pnas.77.3.1374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Olexa S. A., Budzynski A. Z. Localization of a fibrin polymerization site. J Biol Chem. 1981 Apr 10;256(7):3544–3549. [PubMed] [Google Scholar]
  10. Pandya B. V., Cierniewski C. S., Budzynski A. Z. Conservation of human fibrinogen conformation after cleavage of the B beta chain NH2 terminus. J Biol Chem. 1985 Mar 10;260(5):2994–3000. [PubMed] [Google Scholar]
  11. Purves L. R., Lindsey G. G., Franks J. J. Sites of D-domain interaction in fibrin-derived D dimer. Biochemistry. 1980 Aug 19;19(17):4051–4058. doi: 10.1021/bi00558a023. [DOI] [PubMed] [Google Scholar]
  12. Saito Y., Shimizu A., Matsushima A., Inada Y. Chemical modification of fibrinogen and the effect on fibrin formation. Ann N Y Acad Sci. 1983 Jun 27;408:288–300. doi: 10.1111/j.1749-6632.1983.tb23252.x. [DOI] [PubMed] [Google Scholar]
  13. Shainoff J. R., Dardik B. N. Fibrinopeptide B and aggregation of fibrinogen. Science. 1979 Apr 13;204(4389):200–202. doi: 10.1126/science.155308. [DOI] [PubMed] [Google Scholar]
  14. Shimizu A., Saito Y., Matsushima A., Inada Y. Identification of an essential histidine residue for fibrin polymerization. Essential role of histidine 16 of the B beta-chain. J Biol Chem. 1983 Jul 10;258(13):7915–7917. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES