Figure 5.

Luciferase reporter assays of full-length glucocorticoid receptor (GR)α (blue diamonds), truncated GRα-D1 isoform (green squares), and combination of both full-length GRα and truncated GRα-D1 (red triangles). (a) GRα isoform transfections with a narrow range of dexamethasone concentrations—0, 0.1, 1, 10, and 100 nM; (b) GRα isoform transfections with a wider range of dexamethasone concentrations— 0, 10, 50, 100, 500, and 1000 nM. In both assays, the full-length GRα, and the truncated GRα-D1 isoforms increased transcription at glucocorticoid response elements (GREs) in response to dexamethasone, when compared with an empty vector control. No additional effect was observed when co-transfecting both the full-length GRα and truncated GRα-D1 isoforms. Results are the normalised mean of (a) two or (b) four independent experiments, with treatments performed in triplicate in each experiment. Error bars represent SEM.