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. 2011 Nov 14;3(11):2223–2237. doi: 10.3390/v3112223

Table 2.

Oligonucleotides used for myr-add mutagenesis.

Primer Name Sequence a
1 5′-CCGGTACCGCCATGGGCCAGACCGTGACCACCCCCCTGATGGC-3′
2 5′-TCGAGCCATCAGGGGGGTGGTCACGGTCTGGCCCATGGCGGTA-3′
3 5′-CCGGTACCGCCATGGGCTGCGTGCAGTGCAAGGACAAGATGGC-3′
4 5′-TCGAGCCATCTTGTCCTTGCACTGCACGCAGCCCATGGCGGTA-3′
5 5′-CCGGTACCGCCATGGGCTGCGGCTGCAGCAGCCACCCCATGGC-3′
6 5′-TCGAGCCATGGGGTGGCTGCTGCAGCCGCAGCCCATGGCGGTA-3′
7 5′-CCGGTACCGCCATGGGCAGCAGCAAGAGCAAGCCCAAGATGGC-3′
8 5′-TCGAGCCATCTTGGGCTTGCTCTTGCTGCTGCCCATGGCGGTA-3′
a

the AgeI single-stranded overhangs are underlined, those of the XhoI site are in bold.