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. 1986 Mar;83(5):1443–1447. doi: 10.1073/pnas.83.5.1443

Monoclonal antibodies specific for H-2K and H-2D antigens on cytotoxic T cells can inhibit their function.

H C O'Neill
PMCID: PMC323092  PMID: 3006052

Abstract

Antibodies specific for murine major histocompatibility gene complex (MHC) class I H-2K and H-2D molecules present on cytotoxic T (Tc) cells have been shown to inhibit their function of target cell lysis. This could only be demonstrated by using a more sensitive assay for T-cell-mediated lysis, and many monoclonal antibodies of different Ig class, origin, and specificity can be shown to inhibit alloreactive as well as MHC-restricted Tc cells. These antibodies inhibit different activated T-cell populations to varying extents, and anti-H-2K but not anti-H-2D antibodies show a synergistic effect with anti-Lyt-2 antibodies. Data here suggest that MHC molecules may be located in or near the T-cell receptor complex on these cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison J. P., McIntyre B. W., Bloch D. Tumor-specific antigen of murine T-lymphoma defined with monoclonal antibody. J Immunol. 1982 Nov;129(5):2293–2300. [PubMed] [Google Scholar]
  2. Flaherty L., Zimmerman D. Surface mapping of mouse thymocytes. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1990–1993. doi: 10.1073/pnas.76.4.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Lemke H., Hämmerling G. J., Hämmerling U. Fine specificity analysis with monoclonal antibodies of antigens controlled by the major histocompatibility complex and by the Qa/TL region in mice. Immunol Rev. 1979;47:175–206. doi: 10.1111/j.1600-065x.1979.tb00293.x. [DOI] [PubMed] [Google Scholar]
  4. Marrack P., Hannum C., Harris M., Haskins K., Kubo R., Pigeon M., Shimonkevitz R., White J., Kappler J. Antigen-specific, major histocompatibility complex-restricted T cell receptors. Immunol Rev. 1983;76:131–145. doi: 10.1111/j.1600-065x.1983.tb01100.x. [DOI] [PubMed] [Google Scholar]
  5. Mason D. W., Williams A. F. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980 Apr 1;187(1):1–20. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Meuer S. C., Acuto O., Hercend T., Schlossman S. F., Reinherz E. L. The human T-cell receptor. Annu Rev Immunol. 1984;2:23–50. doi: 10.1146/annurev.iy.02.040184.000323. [DOI] [PubMed] [Google Scholar]
  7. Murphy R. F., Tse D. B., Cantor C. R., Pernis B. Acidification of internalized class I major histocompatibility complex antigen by T lymphoblasts. Cell Immunol. 1984 Oct 15;88(2):336–342. doi: 10.1016/0008-8749(84)90166-7. [DOI] [PubMed] [Google Scholar]
  8. Müllbacher A., Parish C. R., Mundy J. P. An improved colorimetric assay for T cell cytotoxicity in vitro. J Immunol Methods. 1984 Mar 30;68(1-2):205–215. doi: 10.1016/0022-1759(84)90151-0. [DOI] [PubMed] [Google Scholar]
  9. O'Neill H. C., Blanden R. V. Quantitative differences in the expression of parentally-derived H-2 antigens in F1 hybrid mice affect T-cell responses. J Exp Med. 1979 Mar 1;149(3):724–731. doi: 10.1084/jem.149.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. O'Neill H. C. Heterogeneity among H-2Kk antigens. Differential expression on lymphoid cells. Transplantation. 1984 Aug;38(2):182–188. [PubMed] [Google Scholar]
  11. O'Neill H. C., Parish C. R. A rapid, automated colorimetric assay for measuring antibody binding to cell surface antigens. J Immunol Methods. 1983 Nov 25;64(3):257–268. doi: 10.1016/0022-1759(83)90433-7. [DOI] [PubMed] [Google Scholar]
  12. Ozato K., Mayer N., Sachs D. H. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980 Feb;124(2):533–540. [PubMed] [Google Scholar]
  13. Ozato K., Sachs D. H. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981 Jan;126(1):317–321. [PubMed] [Google Scholar]
  14. Parish C. R., McKenzie I. F. A sensitive rosetting method for detecting subpopulations of lymphocytes which react with alloantisera. J Immunol Methods. 1978;20:173–183. doi: 10.1016/0022-1759(78)90254-5. [DOI] [PubMed] [Google Scholar]
  15. Sanderson C. J. The mechanism of lymphocyte-mediated cytotoxicity. Biol Rev Camb Philos Soc. 1981 May;56(2):153–197. doi: 10.1111/j.1469-185x.1981.tb00346.x. [DOI] [PubMed] [Google Scholar]
  16. Sitkovsky M. V., Pasternack M. S., Lugo J. P., Klein J. R., Eisen H. N. Isolation and partial characterization of concanavalin A receptors on cloned cytotoxic T lymphocytes. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1519–1523. doi: 10.1073/pnas.81.5.1519. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tse D. B., Pernis B. Spontaneous internalization of Class I major histocompatibility complex molecules in T lymphoid cells. J Exp Med. 1984 Jan 1;159(1):193–207. doi: 10.1084/jem.159.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woolnough J. A., Lafferty K. J. Generation of homogeneous populations of alloreactive T cells in vitro. Aust J Exp Biol Med Sci. 1979 Apr;57(2):127–139. doi: 10.1038/icb.1979.13. [DOI] [PubMed] [Google Scholar]
  19. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]

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