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. 1986 Mar;83(5):1467–1471. doi: 10.1073/pnas.83.5.1467

Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B.

A A Protter, D A Hardman, J W Schilling, J Miller, V Appleby, G C Chen, S W Kirsher, G McEnroe, J P Kane
PMCID: PMC323097  PMID: 3513177

Abstract

A partial cDNA clone for the B-26 region of apolipoprotein B was isolated from an adult human liver DNA library by screening with an oligonucleotide probe derived from amino-terminal protein sequence obtained from purified B-26 peptide. Antisera against a synthetic 17-residue peptide whose amino acid sequence was encoded by the clone cross-reacts with apolipoproteins B-26, B-100, and B-48, but not with B-74. The nucleotide sequence immediately upstream from the amino terminus of B-26 codes for an apparent signal sequence, implying that the B-26 moiety is in an amino-terminal locus in the B-100 protein. That this sequence represents a 5' end region is further supported by primer extension analysis using a fragment of the cDNA clone and by S1 nuclease protection experiments using the corresponding region in a genomic clone.

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Selected References

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