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. 1986 Mar;83(6):1719–1722. doi: 10.1073/pnas.83.6.1719

Visualization of the Ca2+-transport system of the mitotic apparatus of sea urchin eggs with a monoclonal antibody

Christian Petzelt 1, Mathias Hafner 1
PMCID: PMC323155  PMID: 16593667

Abstract

Monoclonal antibodies have been obtained to components of Ca2+-sequestering vesicles from the endoplasmic reticulum of HeLa cells by isolating hybridomas that were generated by the in vitro immunization of lymphocytes followed by fusion with plasmocytoma cells. One of these monoclonal antibodies specifically labels punctate structures which appear in the mitotic apparatus of sea urchin eggs at the beginning of prophase and disappear upon the completion of cytokinesis. The antibody inhibits the Ca2+ uptake of the membrane system in vitro. It reacts with one 46-kDa protein out of the complex protein mixture from the membrane fraction. We take all this as evidence that in fact a specific Ca2+-transport system is part of the mitotic apparatus, that such a system is very conserved, and that it is most probably derived from the endoplasmic reticulum.

Keywords: Ca2+ regulation, mitosis, in vitro immunization

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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