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. 1986 Mar;83(6):1960–1963. doi: 10.1073/pnas.83.6.1960

Proenkephalin mRNA in rat heart.

R D Howells, D L Kilpatrick, L C Bailey, M Noe, S Udenfriend
PMCID: PMC323204  PMID: 3456615

Abstract

The distribution of preproenkephalin mRNA in rat tissues was investigated using a homologous cDNA probe for detection. The heart was found to contain larger amounts of the mRNA than any other tissue including brain, which heretofore had been considered the richest source. The identity of the message in heart was verified by hybridizing RNA blots with a synthetic oligodeoxynucleotide that recognizes a different region of the preproenkephalin mRNA sequence than does the cDNA probe. The preproenkephalin mRNA extracted from both heart and brain contained approximately equal to 1500 bases. Dissection of heart revealed that essentially all of the message is contained within the ventricles. In contrast to the large amounts of preproenkephalin mRNA in rat heart, the opioid peptide contents is only 3% of the amount in brain. The rat heart may be a useful model for the investigation of translation control of protein synthesis.

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Selected References

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  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bailey J. M., Davidson N. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976 Jan;70(1):75–85. doi: 10.1016/s0003-2697(76)80049-8. [DOI] [PubMed] [Google Scholar]
  3. Böhlen P., Stein S., Stone J., Udenfriend S. Automatic Monitoring of primary amines in preparative column effluents with fluorescamine. Anal Biochem. 1975 Aug;67(2):438–445. doi: 10.1016/0003-2697(75)90316-4. [DOI] [PubMed] [Google Scholar]
  4. Chirgwin J. M., Przybyla A. E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979 Nov 27;18(24):5294–5299. doi: 10.1021/bi00591a005. [DOI] [PubMed] [Google Scholar]
  5. Currie M. G., Geller D. M., Cole B. R., Siegel N. R., Fok K. F., Adams S. P., Eubanks S. R., Galluppi G. R., Needleman P. Purification and sequence analysis of bioactive atrial peptides (atriopeptins). Science. 1984 Jan 6;223(4631):67–69. doi: 10.1126/science.6419347. [DOI] [PubMed] [Google Scholar]
  6. Fabbri A., Tsai-Morris C. H., Luna S., Fraioli F., Dufau M. L. Opiate receptors are present in the rat testis. Identification and localization in Sertoli cells. Endocrinology. 1985 Dec;117(6):2544–2546. doi: 10.1210/endo-117-6-2544. [DOI] [PubMed] [Google Scholar]
  7. Glazer E. J., Basbaum A. I. Leucine enkephalin: localization in and axoplasmic transport by sacral parasympathetic preganglionic neurons. Science. 1980 Jun 27;208(4451):1479–1481. doi: 10.1126/science.6155697. [DOI] [PubMed] [Google Scholar]
  8. Howells R. D., Gioannini T. L., Hiller J. M., Simon E. J. Solubilization and characterization of active opiate binding sites from mammalian brain. J Pharmacol Exp Ther. 1982 Sep;222(3):629–634. [PubMed] [Google Scholar]
  9. Howells R. D., Kilpatrick D. L., Bhatt R., Monahan J. J., Poonian M., Udenfriend S. Molecular cloning and sequence determination of rat preproenkephalin cDNA: sensitive probe for studying transcriptional changes in rat tissues. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7651–7655. doi: 10.1073/pnas.81.23.7651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hughes J., Kosterlitz H. W., Smith T. W. The distribution of methionine-enkephalin and leucine-enkephalin in the brain and peripheral tissues. Br J Pharmacol. 1977 Dec;61(4):639–647. doi: 10.1111/j.1476-5381.1977.tb07557.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hökfelt T., Ljungdahl A., Terenius L., Elde R., Nilsson G. Immunohistochemical analysis of peptide pathways possibly related to pain and analgesia: enkephalin and substance P. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3081–3085. doi: 10.1073/pnas.74.7.3081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. JAMIESON J. D., PALADE G. E. SPECIFIC GRANULES IN ATRIAL MUSCLE CELLS. J Cell Biol. 1964 Oct;23:151–172. doi: 10.1083/jcb.23.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kilpatrick D. L., Howells R. D., Noe M., Bailey L. C., Udenfriend S. Expression of preproenkephalin-like mRNA and its peptide products in mammalian testis and ovary. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7467–7469. doi: 10.1073/pnas.82.21.7467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kojima K., Kilpatrick D. L., Stern A. S., Jones B. N., Udenfriend S. Proenkephalin: a general pathway for enkephalin biosynthesis in animal tissues. Arch Biochem Biophys. 1982 May;215(2):638–643. doi: 10.1016/0003-9861(82)90125-4. [DOI] [PubMed] [Google Scholar]
  15. Lahm H. W., Gerber L. D., Brink L., Kilpatrick D. L., Udenfriend S. Specific polyclonal antibodies to the carboxyl terminus of [Met]enkephalin-Arg6-Gly7-Leu8. Arch Biochem Biophys. 1983 Sep;225(2):422–429. doi: 10.1016/0003-9861(83)90049-8. [DOI] [PubMed] [Google Scholar]
  16. Lang R. E., Hermann K., Dietz R., Gaida W., Ganten D., Kraft K., Unger T. Evidence for the presence of enkephalins in the heart. Life Sci. 1983 Jan 24;32(4):399–406. doi: 10.1016/0024-3205(83)90086-3. [DOI] [PubMed] [Google Scholar]
  17. Lewis R. V., Stein S., Gerber L. D., Rubinstein M., Udenfriend S. High molecular weight opioid-containing proteins in striatum. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4021–4023. doi: 10.1073/pnas.75.8.4021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lewis R. V., Stern A. S., Rossier J., Stein S., Udenfriend S. Putative enkephalin precursors in bovine adrenal medulla. Biochem Biophys Res Commun. 1979 Aug 13;89(3):822–829. doi: 10.1016/0006-291x(79)91852-7. [DOI] [PubMed] [Google Scholar]
  19. Linnoila R. I., DiAugustine R. P., Miller R. J., Chang K. J., Cuatrecasas P. An immunohistochemical and radioimmunological study of the distribution of [met5]- and [leu5]-enkephalin in the gastrointestinal tract. Neuroscience. 1978;3(12):1187–1196. doi: 10.1016/0306-4522(78)90138-0. [DOI] [PubMed] [Google Scholar]
  20. Manwell C. Enzyme variability in the protochordate amphioxus. Nature. 1975 Dec 18;258(5536):606–608. doi: 10.1038/258606a0. [DOI] [PubMed] [Google Scholar]
  21. Pintar J. E., Schachter B. S., Herman A. B., Durgerian S., Krieger D. T. Characterization and localization of proopiomelanocortin messenger RNA in the adult rat testis. Science. 1984 Aug 10;225(4662):632–634. doi: 10.1126/science.6740329. [DOI] [PubMed] [Google Scholar]
  22. Saper C. B., Standaert D. G., Currie M. G., Schwartz D., Geller D. M., Needleman P. Atriopeptin-immunoreactive neurons in the brain: presence in cardiovascular regulatory areas. Science. 1985 Mar 1;227(4690):1047–1049. doi: 10.1126/science.2858127. [DOI] [PubMed] [Google Scholar]
  23. Schultzberg M., Hökfelt T., Lundberg J. M., Terenius L., Elfvin L. G., Elde R. Enkephalin-like immunoreactivity in nerve terminals in sympathetic ganglia and adrenal medulla and in adrenal medullary gland cells. Acta Physiol Scand. 1978 Aug;103(4):475–477. doi: 10.1111/j.1748-1716.1978.tb06243.x. [DOI] [PubMed] [Google Scholar]
  24. Schultzberg M., Hökfelt T., Terenius L., Elfvin L. G., Lundberg J. M., Brandt J., Elde R. P., Goldstein M. Enkephalin immunoreactive nerve fibres and cell bodies in sympathetic ganglia of the guinea-pig and rat. Neuroscience. 1979;4(2):249–270. doi: 10.1016/0306-4522(79)90087-3. [DOI] [PubMed] [Google Scholar]
  25. Stern A. S., Wurzburger R. J., Barkey R., Spector S. Opioid polypeptides in guinea pig pancreas. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6703–6706. doi: 10.1073/pnas.79.21.6703. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tang J., Chou J., Zhang A. Z., Yang H. Y., Costa E. Met5-enkephalin-arg6-phe7 and its receptor in lung. Life Sci. 1983 May 16;32(20):2371–2377. doi: 10.1016/0024-3205(83)90768-3. [DOI] [PubMed] [Google Scholar]
  27. Weihe E., McKnight A. T., Corbett A. D., Hartschuh W., Reinecke M., Kosterlitz H. W. Characterization of opioid peptides in guinea-pig heart and skin. Life Sci. 1983;33 (Suppl 1):711–714. doi: 10.1016/0024-3205(83)90601-x. [DOI] [PubMed] [Google Scholar]
  28. Wilson S. P., Klein R. L., Chang K. J., Gasparis M. S., Viveros O. H., Yang W. H. Are opioid peptides co-transmitters in noradrenergic vesicles of sympathetic nerves? Nature. 1980 Dec 25;288(5792):707–709. doi: 10.1038/288707a0. [DOI] [PubMed] [Google Scholar]
  29. Yoshikawa K., Williams C., Sabol S. L. Rat brain preproenkephalin mRNA. cDNA cloning, primary structure, and distribution in the central nervous system. J Biol Chem. 1984 Nov 25;259(22):14301–14308. [PubMed] [Google Scholar]

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