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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 Apr;83(7):2233–2237. doi: 10.1073/pnas.83.7.2233

A newly identified hepatitis B type virus in tree squirrels.

M A Feitelson, I Millman, T Halbherr, H Simmons, B S Blumberg
PMCID: PMC323266  PMID: 3457384

Abstract

Virus-associated particles have been isolated from the livers of three common gray tree squirrels (Sciurus carolinensis pennsylvanicus) that have histological evidence of hepatitis. Two of these livers were also positive by orcein staining, suggesting the presence of surface antigen in the cytoplasm of hepatocytes. Fractionation of these particles by CsCl density equilibrium gradient centrifugation and assay of the fractions for surface antigen, core antigen, and DNA polymerase activities demonstrate the presence of all three at an approximate density peak of 1.27. Electron microscopic examination of purified virus preparations showed spherical particles with a mean diameter of 25 nm. Initial characterization of the DNA polymerase product by gel electrophoresis showed a single DNase I sensitive band, migrating slightly faster than the woodchuck hepatitis virus DNA polymerase product. The presence of apparently cross-reacting antibodies was demonstrated by purified hepatitis B surface and/or core antigens binding to some squirrel sera in solid phase assays. Infected tree squirrels appear to lack detectable antigen in their sera. These results suggest that the tree squirrels studied are chronic carriers of a hepatitis B type virus. The host-virus interaction described herein may be useful in understanding the chronic carrier state associated with hepatitis B in man.

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Selected References

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  1. Andres L. L., Sawhney V. K., Scullard G. H., Smith J. L., Merigan T. C., Robinson W. S., Gregory P. B. Dane particle DNA polymerase and HBeAg: impact on clinical, laboratory, and histologic findings in hepatitis B-associated chronic liver disease. Hepatology. 1981 Nov-Dec;1(6):583–585. doi: 10.1002/hep.1840010604. [DOI] [PubMed] [Google Scholar]
  2. Feitelson M. A., Marion P. L., Robinson W. S. Antigenic and structural relationships of the surface antigens of hepatitis B virus, ground squirrel hepatitis virus, and woodchuck hepatitis virus. J Virol. 1981 Aug;39(2):447–454. doi: 10.1128/jvi.39.2.447-454.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Feitelson M. A., Marion P. L., Robinson W. S. Core particles of hepatitis B virus and ground squirrel hepatitis virus. I. Relationship between hepatitis B core antigen- and ground squirrel hepatitis core antigen-associated polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic peptide mapping. J Virol. 1982 Aug;43(2):687–696. doi: 10.1128/jvi.43.2.687-696.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ganem D., Greenbaum L., Varmus H. E. Virion DNA of ground squirrel hepatitis virus: structural analysis and molecular cloning. J Virol. 1982 Oct;44(1):374–383. doi: 10.1128/jvi.44.1.374-383.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gerlich W. H., Feitelson M. A., Marion P. L., Robinson W. S. Structural relationships between the surface antigens of ground squirrel hepatitis virus and human hepatitis B virus. J Virol. 1980 Dec;36(3):787–795. doi: 10.1128/jvi.36.3.787-795.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lieberman H. M., LaBrecque D. R., Kew M. C., Hadziyannis S. J., Shafritz D. A. Detection of hepatitis B virus DNA directly in human serum by a simplified molecular hybridization test: comparison to HBeAg/anti-HBe status in HBsAg carriers. Hepatology. 1983 May-Jun;3(3):285–291. doi: 10.1002/hep.1840030302. [DOI] [PubMed] [Google Scholar]
  7. Marion P. L., Oshiro L. S., Regnery D. C., Scullard G. H., Robinson W. S. A virus in Beechey ground squirrels that is related to hepatitis B virus of humans. Proc Natl Acad Sci U S A. 1980 May;77(5):2941–2945. doi: 10.1073/pnas.77.5.2941. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Marion P. L., Robinson W. S. Hepadna viruses: hepatitis B and related viruses. Curr Top Microbiol Immunol. 1983;105:99–121. doi: 10.1007/978-3-642-69159-1_2. [DOI] [PubMed] [Google Scholar]
  9. Mason W. S., Seal G., Summers J. Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J Virol. 1980 Dec;36(3):829–836. doi: 10.1128/jvi.36.3.829-836.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller R. H., Robinson W. S. Hepatitis B virus DNA forms in nuclear and cytoplasmic fractions of infected human liver. Virology. 1984 Sep;137(2):390–399. doi: 10.1016/0042-6822(84)90231-9. [DOI] [PubMed] [Google Scholar]
  11. Musca A., Cordova C., Barnaba V., Bonavita M. S., Levrero M., Zaccari C., Balsano F. HBeAg/anti-HBe system and development of primary hepatocellular carcinoma in patients with HBsAg-positive liver cirrhosis. Hepatogastroenterology. 1983 Feb;30(1):3–5. [PubMed] [Google Scholar]
  12. Omata M., Uchiumi K., Ito Y., Yokosuka O., Mori J., Terao K., Wei-Fa Y., O'Connell A. P., London W. T., Okuda K. Duck hepatitis B virus and liver diseases. Gastroenterology. 1983 Aug;85(2):260–267. [PubMed] [Google Scholar]
  13. Onodera S., Ohori H., Yamaki M., Ishida N. Electron microscopy of human hepatitis B virus cores by negative staining-carbon film technique. J Med Virol. 1982;10(2):147–155. doi: 10.1002/jmv.1890100209. [DOI] [PubMed] [Google Scholar]
  14. Robinson W. S. DNA and DNA polymerase in the core of the Dane particle of hepatitis B. Am J Med Sci. 1975 Jul-Aug;270(1):151–159. doi: 10.1097/00000441-197507000-00021. [DOI] [PubMed] [Google Scholar]
  15. Robinson W. S., Greenman R. L. DNA polymerase in the core of the human hepatitis B virus candidate. J Virol. 1974 Jun;13(6):1231–1236. doi: 10.1128/jvi.13.6.1231-1236.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shikata T., Uzawa T., Yoshiwara N., Akatsuka T., Yamazaki S. Staining methods of Australia antigen in paraffin section--detection of cytoplasmic inclusion bodies. Jpn J Exp Med. 1974 Feb;44(1):25–36. [PubMed] [Google Scholar]
  17. Summers J., Mason W. S. Replication of the genome of a hepatitis B--like virus by reverse transcription of an RNA intermediate. Cell. 1982 Jun;29(2):403–415. doi: 10.1016/0092-8674(82)90157-x. [DOI] [PubMed] [Google Scholar]
  18. Summers J., Smolec J. M., Snyder R. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4533–4537. doi: 10.1073/pnas.75.9.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Werner B. G., Murphy B. L., Maynard J. E., Larouzé B. Anti-e in primary hepatic carcinoma. Lancet. 1976 Mar 27;1(7961):696–696. doi: 10.1016/s0140-6736(76)92813-0. [DOI] [PubMed] [Google Scholar]

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