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. 1986 Apr;83(8):2335–2339. doi: 10.1073/pnas.83.8.2335

Cloning and expression of human lecithin-cholesterol acyltransferase cDNA.

J McLean, C Fielding, D Drayna, H Dieplinger, B Baer, W Kohr, W Henzel, R Lawn
PMCID: PMC323291  PMID: 3458198

Abstract

cDNA and genomic cloning has been used to determine the mRNA and amino acid sequence of human plasma lecithin-cholesterol acyltransferase (LCATase; EC 2.3.1.43). The mature protein was found to contain 416 amino acid residues with a hydrophobic leader sequence of 24 amino acids. An unusual feature of the message is that the poly(A) signal AATAAA overlaps the COOH-terminal glutamic acid and stop codons, and the 3' untranslated region is only 23 bases. The protein itself is distinguished by a number of extended sequences of hydrophobic amino acids, one of which contains a hexapeptide identical with the interfacial binding segment of the active site of pancreatic lipase and is similar to the same site of lingual lipase. The cloned cDNA allows the expression of active LCATase by transfected tissue culture cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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