Inverse correlation between expression of TCL1 and PTPROt. Whole cell extracts of primary CLL cells were subjected to Western blot analysis with antibodies against TCL1, c-fos, c-jun, p(S73)-c-jun, and GAPDH (normalizer). The autoradiographs were scanned and signal intensities were quantified using AlphaImager. Total RNA from the same samples was used for real-time RT-PCR analysis of PTPROt and GAPDH (normalizer). Each assay was performed in triplicate. Linear regression model was used for statistical correlation between normalized expression values for PTPROt mRNA and TCL1 protein (A), p-c-jun (B), or c-fos (C).