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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 May;83(10):3267–3271. doi: 10.1073/pnas.83.10.3267

Distribution of ions around DNA, probed by energy transfer.

T G Wensel, C F Meares, V Vlachy, J B Matthew
PMCID: PMC323494  PMID: 3458180

Abstract

Measurements of the effect of DNA on rates of bimolecular energy transfer between ions provide a direct indication of how cations cluster in regions near DNA and how anions are repelled from the same regions. Energy transfer from luminescent lanthanide ions (in the "rapid-diffusion" limit) probes collision frequencies that are dependent on the equilibrium spatial distributions of ions. The addition of 1 mM DNA (phosphate) to a 2 mM salt solution increases the overall collision frequency between monovalent cations by a factor of 6 +/- 1.5; it increases the divalent-monovalent cation collision frequency by a factor of 29 +/- 3; and it decreases the divalent cation-monovalent anion collision frequency by a factor of 0.24 +/- 0.03. Comparisons are made with the changes in collision frequencies predicted by several different theoretical descriptions of ion distributions. The closest agreement with experimental results for monovalent ions at 1 mM DNA is obtained with a static accessibility-modified discrete charge calculation, based on a detailed molecular model of B-DNA. At high DNA concentration (10 mM), the best results are obtained by numerical solutions of the Poisson-Boltzmann equation for a "soft-rod" model of DNA. Poisson-Boltzmann calculations for a "hard-rod" model greatly overestimate the effects of DNA on collision frequencies, as does a calculation based on counterion-condensation theory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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