Abstract
We report on the characterization of five third chromosome mutations with strong effects on the formation of the eggshell or chorion. Three mutations, defining two loci, result in substantially reduced follicle cell-specific amplification of the major chorion structural genes and, hence, in underproduction of the corresponding mRNAs and proteins. The other two mutations, though displaying structural chorion abnormalities, appear to have no significant effect on amplification and to express normally the major chorion structural genes. The possible nature of these mutations is discussed.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Boyd J. B., Golino M. D., Nguyen T. D., Green M. M. Isolation and characterization of X-linked mutants of Drosophila melanogaster which are sensitive to mutagens. Genetics. 1976 Nov;84(3):485–506. doi: 10.1093/genetics/84.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Church G. M., Gilbert W. Genomic sequencing. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991–1995. doi: 10.1073/pnas.81.7.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gans M., Audit C., Masson M. Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster. Genetics. 1975 Dec;81(4):683–704. doi: 10.1093/genetics/81.4.683. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gatti M., Smith D. A., Baker B. S. A gene controlling condensation of heterochromatin in Drosophila melanogaster. Science. 1983 Jul 1;221(4605):83–85. doi: 10.1126/science.6407113. [DOI] [PubMed] [Google Scholar]
- Griffin-Shea R., Thireos G., Kafatos F. C. Organization of a cluster of four chorion genes in Drosophila and its relationship to developmental expression and amplification. Dev Biol. 1982 Jun;91(2):325–336. doi: 10.1016/0012-1606(82)90039-2. [DOI] [PubMed] [Google Scholar]
- Kalfayan L., Wensink P. C. alpha-Tubulin genes of Drosophila. Cell. 1981 Apr;24(1):97–106. doi: 10.1016/0092-8674(81)90505-5. [DOI] [PubMed] [Google Scholar]
- Komitopoulou K., Gans M., Margaritis L. H., Kafatos F. C., Masson M. Isolation and Characterization of Sex-Linked Female-Sterile Mutants in DROSOPHILA MELANOGASTER with Special Attention to Eggshell Mutants. Genetics. 1983 Dec;105(4):897–920. doi: 10.1093/genetics/105.4.897. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Margaritis L. H., Kafatos F. C., Petri W. H. The eggshell of Drosophila melanogaster. I. Fine structure of the layers and regions of the wild-type eggshell. J Cell Sci. 1980 Jun;43:1–35. doi: 10.1242/jcs.43.1.1. [DOI] [PubMed] [Google Scholar]
- McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orr W., Komitopoulou K., Kafatos F. C. Mutants suppressing in trans chorion gene amplification in Drosophila. Proc Natl Acad Sci U S A. 1984 Jun;81(12):3773–3777. doi: 10.1073/pnas.81.12.3773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Osheim Y. N., Miller O. L., Jr Novel amplification and transcriptional activity of chorion genes in Drosophila melanogaster follicle cells. Cell. 1983 Jun;33(2):543–553. doi: 10.1016/0092-8674(83)90435-x. [DOI] [PubMed] [Google Scholar]
- Petri W. H., Wyman A. R., Kafatos F. C. Specific protein synthesis in cellular differentiation. III. The eggshell proteins of Drosophila melanogaster and their program of synthesis. Dev Biol. 1976 Mar;49(1):185–199. doi: 10.1016/0012-1606(76)90266-9. [DOI] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Smith P. D. Mutagen sensitivity of Drosophila melanogaster. III. X-linked loci governing sensitivity to methyl methanesulfonate. Mol Gen Genet. 1976 Nov 24;149(1):73–85. doi: 10.1007/BF00275962. [DOI] [PubMed] [Google Scholar]
- Spradling A. C., Digan M. E., Mahowald A. P., Scott M., Craig E. A. Two clusters of genes for major chorion proteins of Drosophila melanogaster. Cell. 1980 Apr;19(4):905–914. doi: 10.1016/0092-8674(80)90082-3. [DOI] [PubMed] [Google Scholar]
- Spradling A. C., Mahowald A. P. A chromosome inversion alters the pattern of specific DNA replication in Drosophila follicle cells. Cell. 1981 Nov;27(1 Pt 2):203–209. doi: 10.1016/0092-8674(81)90374-3. [DOI] [PubMed] [Google Scholar]
- Spradling A. C., Mahowald A. P. Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096–1100. doi: 10.1073/pnas.77.2.1096. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spradling A. C. The organization and amplification of two chromosomal domains containing Drosophila chorion genes. Cell. 1981 Nov;27(1 Pt 2):193–201. doi: 10.1016/0092-8674(81)90373-1. [DOI] [PubMed] [Google Scholar]
- Spradling A. C., Waring G. L., Mahowald A. P. Drosophila bearing the ocelliless mutation underproduce two major chorion proteins both of which map near this gene. Cell. 1979 Mar;16(3):609–616. doi: 10.1016/0092-8674(79)90034-5. [DOI] [PubMed] [Google Scholar]
- Thireos G., Shea R. G., Kafatos F. C. Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6279–6283. doi: 10.1073/pnas.76.12.6279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waring G. L., Mahowald A. P. Identification and time of synthesis of chorion proteins in Drosophila melanogaster. Cell. 1979 Mar;16(3):599–607. doi: 10.1016/0092-8674(79)90033-3. [DOI] [PubMed] [Google Scholar]
- Yannoni C. Z., Petri W. H. Localization of a gene for a minor chorion protein in Drosophila melanogaster: a new chorion structural locus. Dev Biol. 1984 Apr;102(2):504–508. doi: 10.1016/0012-1606(84)90217-3. [DOI] [PubMed] [Google Scholar]
- de Cicco D. V., Spradling A. C. Localization of a cis-acting element responsible for the developmentally regulated amplification of Drosophila chorion genes. Cell. 1984 Aug;38(1):45–54. doi: 10.1016/0092-8674(84)90525-7. [DOI] [PubMed] [Google Scholar]