Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1986 May;83(10):3381–3385. doi: 10.1073/pnas.83.10.3381

The qa repressor gene of Neurospora crassa: wild-type and mutant nucleotide sequences.

L Huiet, N H Giles
PMCID: PMC323517  PMID: 3010294

Abstract

The qa-1S gene, one of two regulatory genes in the qa gene cluster of Neurospora crassa, encodes the qa repressor. The qa-1S gene together with the qa-1F gene, which encodes the qa activator protein, control the expression of all seven qa genes, including those encoding the inducible enzymes responsible for the utilization of quinic acid as a carbon source. The nucleotide sequence of the qa-1S gene and its flanking regions has been determined. The deduced coding sequence for the qa-1S protein encodes 918 amino acids with a calculated molecular weight of 100,650 and is interrupted by a single 66-base-pair intervening sequence. Both constitutive and noninducible mutants occur in the qa-1S gene and two different mutations of each type have been cloned and sequenced. All four mutations occur within the predicted coding region of the qa-1S gene. This result strongly supports the hypothesis that the qa-1S gene encodes a repressor. All four mutations are located within codons for the last 300 amino acids of the qa-1S protein. The mutations in three of the mutants involve amino acid substitutions, while the fourth mutant, which has a constitutive phenotype, contains a frameshift mutation. The two constitutive mutations occur in the most distal region of the gene, possibly implicating the COOH-terminal region of the qa repressor in binding to its target. The two noninducible mutations occur in a region proximal to the constitutive mutations, possibly implicating this region of the qa repressor in binding the inducer.

Full text

PDF
3381

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alton N. K., Buxton F., Patel V., Giles N. H., Vapnek D. 5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1955–1959. doi: 10.1073/pnas.79.6.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arends H., Sebald W. Nucleotide sequence of the cloned mRNA and gene of the ADP/ATP carrier from Neurospora crassa. EMBO J. 1984 Feb;3(2):377–382. doi: 10.1002/j.1460-2075.1984.tb01815.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennetzen J. L., Hall B. D. Codon selection in yeast. J Biol Chem. 1982 Mar 25;257(6):3026–3031. [PubMed] [Google Scholar]
  4. Berk A. J., Sharp P. A. Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274–1278. doi: 10.1073/pnas.75.3.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Case M. E., Giles N. H. Genetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa. Proc Natl Acad Sci U S A. 1975 Feb;72(2):553–557. doi: 10.1073/pnas.72.2.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Favaloro J., Treisman R., Kamen R. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 1980;65(1):718–749. doi: 10.1016/s0076-6879(80)65070-8. [DOI] [PubMed] [Google Scholar]
  7. Fischer H. D., Dodgson J. B., Hughes S., Engel J. D. An unusual 5' splice sequence is efficiently utilized in vivo. Proc Natl Acad Sci U S A. 1984 May;81(9):2733–2737. doi: 10.1073/pnas.81.9.2733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Frischauf A. M., Lehrach H., Poustka A., Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. doi: 10.1016/s0022-2836(83)80190-9. [DOI] [PubMed] [Google Scholar]
  9. Giles N. H., Case M. E., Baum J., Geever R., Huiet L., Patel V., Tyler B. Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa. Microbiol Rev. 1985 Sep;49(3):338–358. doi: 10.1128/mr.49.3.338-358.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Huiet L. Molecular analysis of the Neurospora qa-1 regulatory region indicates that two interacting genes control qa gene expression. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1174–1178. doi: 10.1073/pnas.81.4.1174. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kinnaird J. H., Fincham J. R. The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene. Gene. 1983 Dec;26(2-3):253–260. doi: 10.1016/0378-1119(83)90195-6. [DOI] [PubMed] [Google Scholar]
  12. Kozak M. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984 Jan 25;12(2):857–872. doi: 10.1093/nar/12.2.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laughon A., Gesteland R. F. Primary structure of the Saccharomyces cerevisiae GAL4 gene. Mol Cell Biol. 1984 Feb;4(2):260–267. doi: 10.1128/mcb.4.2.260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  15. Miller J. H. Genetic studies of the lac repressor. XI. On aspects of lac repressor structure suggested by genetic experiments. J Mol Biol. 1979 Jun 25;131(2):249–258. doi: 10.1016/0022-2836(79)90075-5. [DOI] [PubMed] [Google Scholar]
  16. Nogi Y., Fukasawa T. Nucleotide sequence of the yeast regulatory gene GAL80. Nucleic Acids Res. 1984 Dec 21;12(24):9287–9298. doi: 10.1093/nar/12.24.9287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Partridge C. W., Case M. E., Giles N. H. Direct induction in wild-type Neurospora crassa of mutants (qa-1 c ) constitutive for the catabolism of quinate and shikimate. Genetics. 1972 Nov;72(3):411–417. doi: 10.1093/genetics/72.3.411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Patel V. B., Giles N. H. Autogenous regulation of the positive regulatory qa-1F gene in Neurospora crassa. Mol Cell Biol. 1985 Dec;5(12):3593–3599. doi: 10.1128/mcb.5.12.3593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rines H. W., Case M. E., Giles N. H. Mutants in the arom gene cluster of Neurospora crassa specific for biosynthetic dehydroquinase. Genetics. 1969 Apr;61(4):789–800. doi: 10.1093/genetics/61.4.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rutledge B. J. Molecular characterization of the qa-4 gene of Neurospora crassa. Gene. 1984 Dec;32(3):275–287. doi: 10.1016/0378-1119(84)90003-9. [DOI] [PubMed] [Google Scholar]
  21. Schechtman M. G., Yanofsky C. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli. J Mol Appl Genet. 1983;2(1):83–99. [PubMed] [Google Scholar]
  22. Schweizer M., Case M. E., Dykstra C. C., Giles N. H., Kushner S. R. Identification and characterization of recombinant plasmids carrying the complete qa gene cluster from Neurospora crassa including the qa-1+ regulatory gene. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5086–5090. doi: 10.1073/pnas.78.8.5086. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Treisman R., Proudfoot N. J., Shander M., Maniatis T. A single-base change at a splice site in a beta 0-thalassemic gene causes abnormal RNA splicing. Cell. 1982 Jul;29(3):903–911. doi: 10.1016/0092-8674(82)90452-4. [DOI] [PubMed] [Google Scholar]
  24. Tyler B. M., Geever R. F., Case M. E., Giles N. H. Cis-acting and trans-acting regulatory mutations define two types of promoters controlled by the qa-1F gene of Neurospora. Cell. 1984 Feb;36(2):493–502. doi: 10.1016/0092-8674(84)90242-3. [DOI] [PubMed] [Google Scholar]
  25. Valone J. A., Jr, Case M. E., Giles N. H. Constitutive mutants in a regulatory gene exerting positive control of quinic acid catabolism in Neurospora crassa. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1555–1559. doi: 10.1073/pnas.68.7.1555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Viebrock A., Perz A., Sebald W. The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA. EMBO J. 1982;1(5):565–571. doi: 10.1002/j.1460-2075.1982.tb01209.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Woudt L. P., Pastink A., Kempers-Veenstra A. E., Jansen A. E., Mager W. H., Planta R. J. The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences. Nucleic Acids Res. 1983 Aug 25;11(16):5347–5360. doi: 10.1093/nar/11.16.5347. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES