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. 1986 May;83(10):3465–3468. doi: 10.1073/pnas.83.10.3465

Location of the major antigenic sites involved in rotavirus serotype-specific neutralization.

M L Dyall-Smith, I Lazdins, G W Tregear, I H Holmes
PMCID: PMC323536  PMID: 2422651

Abstract

Antigenic sites on the rotavirus major outershell glycoprotein were identified by using mutant viruses selected for resistance to neutralizing (serotype-specific) monoclonal antibodies. The glycoprotein genes from these mutants were sequenced to determine the position and nature of the resultant amino acid substitutions in the protein. Three regions (A, B, and C) were identified (amino acids 87-96, 145-150, and 211-223, respectively), of which region C appears to be the most important. A mutation in region C caused a 10-fold increase in resistance to neutralization by polyclonal antiviral antiserum. The results of this study, together with other data, indicate that the three-dimensional folding of the native protein is such that regions A and C are in close proximity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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